Small peptide powder and heme peptide red compound medicine for preventing and treating anemia as well as preparation method and application of small peptide powder and heme peptide red compound medicine

A technology of heme and compound preparations, which is applied in the field of medicine, can solve the problems of low absorption rate, achieve low preparation costs, clear targets, and improve the effect of micro-ecological balance

Pending Publication Date: 2022-03-22
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(2) Inorganic iron from plant food is easily interfered by phytic acid, oxalic

Method used

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  • Small peptide powder and heme peptide red compound medicine for preventing and treating anemia as well as preparation method and application of small peptide powder and heme peptide red compound medicine
  • Small peptide powder and heme peptide red compound medicine for preventing and treating anemia as well as preparation method and application of small peptide powder and heme peptide red compound medicine
  • Small peptide powder and heme peptide red compound medicine for preventing and treating anemia as well as preparation method and application of small peptide powder and heme peptide red compound medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1. A method for extracting peptide red compound preparation from animal blood products, comprising the steps of:

[0053] (1) Preparation of small peptide powder

[0054] Take 10 kg of hemoglobin powder, dissolve it with alkaline aqueous solution to make a mixed solution with a concentration of 5% by mass of hemoglobin powder, adjust the pH to pH 10.0, and then stir and dissolve at 50°C for 1 hour. Then add the compound protease by 0.4% of the weight of the raw material hemoglobin powder (specifically: mix the compound protease accounting for 0.4% of the weight of the hemoglobin powder and 50°C pure water with a solid-to-liquid ratio of 1g:10mL, stir evenly and add ), enzymatic hydrolysis at 50°C for 2h; then adjust the pH to pH3, and then continue to enzymatically hydrolyze at 50°C for 1h. Subsequently, the enzymatic hydrolyzate was centrifuged (3500 rpm, 5 min) to obtain 110 L of supernatant and heme-enriched precipitate (wet weight: 20 kg). Then, the 110...

Embodiment 2

[0059] Embodiment 2. A method for extracting peptide red compound preparation from animal blood products, comprising the steps of:

[0060] (1) Preparation of small peptide powder

[0061] Take 10 kg of hemoglobin powder, dissolve it with alkaline aqueous solution to make a mixed solution with a mass percentage concentration of hemoglobin powder, adjust the pH to pH 11.0, and then stir and dissolve at 50°C for 1 hour. Then add the compound protease by 0.8% of the weight of the raw material hemoglobin powder (specifically: mix the compound protease accounting for 0.8% of the weight of the hemoglobin powder and 55°C pure water with a solid-to-liquid ratio of 1g:10mL, stir evenly and add ), enzymatic hydrolysis at 55°C for 6h; then adjust the pH to pH4, and then continue to enzymatically hydrolyze at 55°C for 2h. Then the enzymatic solution was centrifuged (4000 rpm, 10 min) to obtain 190 L of supernatant and heme-enriched precipitate (wet weight: 25 kg). Then, the 190L superna...

Embodiment 3

[0066] The effect of the peptide red compound preparation prepared in embodiment 3, embodiment 1 on model rat hemoglobin (Hb) level

[0067] The test animals were 40 SPF grade SD rats (6 weeks old) (provided by Liaoning Changsheng Biotechnology Co., Ltd.). Drinking water and eating freely, the temperature in the feeding room was kept at 25±1°C, and the light-dark cycle was 12 hours.

[0068] (1) Grouping and administration

[0069] 40 rats were equally divided into 5 groups: blank control group (abbreviated as "blank group"), model group, small peptide powder (prepared by step (1) of embodiment 1) group, heme (prepared by step (1) of embodiment 1) 2) Prepare the crude heme and calculate the dose according to the crude product, the same below, no more details) group and peptide red compound preparation group. Rats in each group were administered from the first day of the experiment. The blank control group was administered with normal saline, the small peptide powder group wa...

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Abstract

The invention belongs to the technical field of medicines, and particularly discloses a preparation method of a peptide red compound preparation prepared from small peptide powder and heme and application of the peptide red compound preparation in preparation of medicines for preventing and treating hemorrhagic anemia. The peptide red compound preparation is prepared by mixing small peptide powder from animal blood products with heme, and experiments prove that the peptide red compound preparation has better effects of softening and moistening intestines and viscera, nourishing yin and blood and the like, and can obviously increase the number of hemoglobin, erythropoietin and erythrocytes of hemorrhagic anemia model animals; the traditional Chinese medicine composition has an obvious enrichment effect and a growth promoting effect on probiotics such as bacillus subtilis and saccharomycetes, is beneficial to maintaining the microecology of intestinal flora, and achieves the effect of treating anemia. The preparation for treating anemia has the advantages of definite effective components, definite curative effect and low price, and the related extraction method is easy to implement and wide in application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a small peptide powder and heme peptide red compound drug for preventing and treating anemia based on "softening intestines, nourishing yin and nourishing blood", its preparation method and application. Background technique [0002] Anemia refers to the reduction of peripheral blood red blood cell volume, which is lower than the lower limit of the normal range, and clinical symptoms such as dizziness, fatigue, drowsiness, and pale complexion appear. Anemia is one of the common complications of inflammatory bowel disease, with an incidence rate between 6.2% and 73.7%. Studies have found that the prevalence of anemia in outpatients with inflammatory bowel disease is 16%, while that in inpatients is as high as 68%. Furthermore, scientists had thought that blood cells were produced exclusively in bones by a special population of hematopoietic stem cells. However, in a n...

Claims

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Application Information

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IPC IPC(8): C12P1/06C07K1/34A61K38/01A61K38/42A61P7/06
CPCC12P1/06C07K1/34A61K38/42A61K38/017A61P7/06A61K2300/00
Inventor 林亲雄李玉桑蒙莎莎李婷婷唐和斌
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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