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Method for controlling a sequencing device

A technology for sequencing and sequencing data, applied in computer security devices, biochemical equipment and methods, sequence analysis, etc., can solve the problems of high quality sequencing, such as expensive, limited, assay availability, sequencing run time, preparation time and cost constraints

Pending Publication Date: 2022-03-22
LIFE TECH CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, use of sequencing can be limited by assay availability, sequencing run time, preparation time, and cost
Additionally, mass sequencing has historically been an expensive process, thus limiting its practice

Method used

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  • Method for controlling a sequencing device
  • Method for controlling a sequencing device
  • Method for controlling a sequencing device

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Embodiment Construction

[0020] As used herein, DNA (deoxyribonucleic acid) can be referred to as a chain of nucleotides consisting of 4 types of nucleotides; A (adenine), T (thymine), C (cytosine) and G (guanine). Purine), and the RNA (ribonucleic acid) contains 4 types of nucleotides; A, U (uracil), G and C. Certain pairs of nucleotides bind specifically to each other in a complementary manner (known as complementary base pairing). That is, adenine (A) pairs with thymine (T) (however, in the case of RNA, adenine (A) pairs with uracil (U)), and cytosine (C) pairs with guanine (G )pair. When a first nucleic acid strand binds to a second nucleic acid strand composed of nucleotides complementary to nucleotides in the first strand, the two strands combine to form a double strand. In various embodiments, "nucleic acid sequencing data", "nucleic acid sequencing information", "nucleic acid sequence", "genome sequence", "gene sequence" or "fragment sequence", "nucleic acid sequence reads" or "nucleic acid ...

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Abstract

Methods and systems are described for controlling sequencing devices and processing sequencing data resulting from sequencing run of assays using cloud-based resources and assay definition files of a local server system. A method may include receiving, at a local server system, an assay definition file from a server of a cloud computing and storage system. The assay definition file may include a code module for configuring the assay. The code module may be stored in a memory of the local server system. The server system may receive sequencing data from a sequencing device. The sequencing device may generate the sequencing data during a sequencing run performed on the assay. The server system may apply an analysis pipeline for the assay to the sequencing data. The analysis pipeline includes an analysis step executed in accordance with the code module from the assay definition file to generate an assay analysis result.

Description

[0001] Related Application Cross Reference [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 889,109, filed August 20, 2019, and U.S. Provisional Application No. 62 / 704,806, filed May 29, 2020, under 35 U.S.C. § 119(e). The entire contents of the aforementioned applications are incorporated herein by reference. technical field [0003] The present disclosure relates to the control of sequencing devices for next-generation sequencing (NGS), including the digital delivery of modular software components containing assay workflows from cloud-based computing and storage system resources. Background technique [0004] Biological and medical research is increasingly turning to nucleic acid sequencing for enhanced biological research and medicine. For example, biologists and zoologists are turning to sequencing to study animal migration, species evolution and the origin of traits. The medical community is using sequencing to study the cause of di...

Claims

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Application Information

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IPC IPC(8): G16B20/30
CPCG16B20/30G16B50/30G16B25/20G16B30/00G16H40/67G16H40/40G16H40/63G16B30/10G16B50/00C12Q1/6869G06F21/6218
Inventor J·高V·库德林加D·莱伊
Owner LIFE TECH CORP