Improved intravenous lining stent rat orthotopic liver transplantation model establishing method

Abstract

The invention discloses an improved vein-lined stent rat orthotopic liver transplantation model establishment method, which is characterized in that an improved vein-lined stent is used for performing anastomosis on SHVC (supravena cava and supravena cava), and compared with an existing SHVC suture technology and an SHVC oversleeve anastomosis technology, the improved vein-lined stent has the advantages that the operation difficulty can be reduced, the operation time can be shortened, the liver-free stage can be controlled within 10 minutes, and the model has the advantages of being simple in structure, convenient to operate and high in efficiency. And the blood coagulation risk can be reduced.

Description

technical field [0001] The invention belongs to the field of animal model making, and in particular relates to a method for establishing an improved veno-lined scaffold rat orthotopic liver transplantation model. Background technique [0002] Orthotopic liver transplantation in rats is an extremely important animal model for studying liver immune rejection, immune tolerance, and ischemia-reperfusion injury; the method of orthotopic liver transplantation in rats is similar to that of human clinical liver transplantation, but due to the complicated operation, The mortality rate is high, and it has not been promoted; since the first successful modeling of rat liver transplantation, rat orthotopic liver transplantation has been continuously studied, and it is also continuously improved and optimized; especially the application of sleeve technology , which reduces the difficulty of orthotopic liver transplantation in rats and increases the success rate; [0003] At present, the ...

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Problems solved by technology

[0002] Orthotopic liver transplantation in rats is an extremely important animal model for studying liver immune rejection, immune tolerance, and ischemia-reperfusion injury; the method of orthotopic liver transplantation in rats is similar to that of human clinical liver transplantation, but due to the complicated operation, The mortality rate is high, and it has not been promoted; since the first successful modeling of rat liver transplantation, rat orthotopic liver transplantation has been continuously studied, and it is also continuously improved and optimized; especially the application of sleeve technology , which reduces the difficulty of orthotopic liver transplantation in rats and increases the success rate;

[0003] At present, the two methods commonly used in rat orthotopic liver transplantation are the "two-cuff" and "three-cuff" methods; without considering the reconstruction of the hepatic artery, the biggest difference between the two transplantation methods lies in the upper and lower parts of the liver. The methods of anastomosis of the suprahepatic venacava (SHVC): the SHVC suture technique of the "two-cuff" method and the SHVC cuff anastomosis technique of the "three-cuff" method have their own advantages and disadvantages; the SHVC suture technique can be better The physiological structure of blood vessels is restored, but there are problems such as difficult suture technique and long anhepatic period; SHVC cuff anastomosis technique can effectively shorten the anhepatic period, but there are difficulties in making the SHVC cuff and many postoperative complications (SHVC injury, kinks and liver damage, etc.)

The veno-lined stent technology invented by researchers in this field can skillfully avoid complicated surgical steps such as SHVC suturing and SHVC cuff making, which greatly reduces the difficulty of transplantation, but this technology still has surgical operations Complicated, long anhepatic period and other problems

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