Injectable artificial dermis for promoting wound healing as well as preparation method and application of injectable artificial dermis

A wound healing, artificial technology, applied in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of affecting the blood supply of the wound, internal curvature, narrowness, complex shape, etc. The effect of forming and promoting tissue repair

Pending Publication Date: 2022-03-25
SHENZHEN QIKANG MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to deep tissue infection and necrosis, and incomplete removal of necrotic tissue, in addition to its complex shape, long and narrow interior, many branches, involving different tissue levels, affecting the blood supply of the wound, making the sinus wall fibrous organization lead to repeated infection of the wound, bacterial organisms Membrane formation, protracted and unhealed, has become a difficult point in clinical treatment
Such chronic wound extensions with dead space and sinus tracts are irregular in shape, flaky artificial dermis is difficult to use, and the filling effect is also poor
[0006] On the other hand, the energy supply at the regeneration site of the wound is a key factor affecting wound healing. Chronic wounds that are difficult to heal often have problems such as insufficient local blood supply and nutritional barriers, which lead to insufficient energy supply. Therefore, the vascularization cycle of the artificial dermis increases, which brings great benefits to patients and hospitals. Increased medical burden

Method used

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  • Injectable artificial dermis for promoting wound healing as well as preparation method and application of injectable artificial dermis
  • Injectable artificial dermis for promoting wound healing as well as preparation method and application of injectable artificial dermis
  • Injectable artificial dermis for promoting wound healing as well as preparation method and application of injectable artificial dermis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] This embodiment provides an injectable artificial dermis that promotes wound healing, and the preparation method is as follows:

[0050] (1) Sodium polyphosphate (Na-polyP) is dissolved in ultrapure water, 20g of 0.05wt% sodium polyphosphate aqueous solution is added to 50g mass fraction and is 1.0wt% collagen aqueous solution, stir well, adjust with 0.1M NaOH The pH is 9.

[0051] (2) 3g chondroitin sulfate dissolved in 30mL mass fraction is 0.1wt% CaCl 2 In the aqueous solution, it is miscible with 70mL ethanol / acetone (1:2v / v) mixture, and fully stirred.

[0052] (3) Inject the collagen-containing solution into the chondroitin sulfate-containing solution at a rate of 10 μL / s using a micro-injector, and stir for 24 hours to form a uniform emulsion. Add glutaraldehyde solution for crosslinking for 30min (glutaraldehyde content is 0.01%).

[0053] (4) The hydrogel microspheres were collected by centrifugation, and the hydrogel microspheres were washed 6 times with ul...

Embodiment 2

[0056] This embodiment provides an injectable artificial dermis that promotes wound healing, and the preparation method is as follows:

[0057] (1) Dissolve sodium polyphosphate (Na-polyP) in ultrapure water, add 20g 0.25wt% sodium polyphosphate aqueous solution to 50g mass fraction of 1.0wt% collagen aqueous solution, stir evenly, adjust pH with 0.1M NaOH for 10.

[0058] (2) 3g chitosan is dissolved in the ZnCl that 30mL mass fraction is 0.1wt% 2 In the aqueous solution, it is miscible with 70mL ethanol / acetone (1:2v / v) mixture, and fully stirred.

[0059] (3) Inject the collagen-containing solution into the chitosan-containing solution at a rate of 20 μL / s using a micro-injector, and stir for 18 hours to form a uniform emulsion. Add genipin solution for cross-linking for 1 h (content: 0.1%).

[0060] (4) The hydrogel microspheres were collected by centrifugation, and the hydrogel microspheres were washed 6 times with ultrapure water to remove the residual cross-linking a...

Embodiment 3

[0063] This embodiment provides an injectable artificial dermis that promotes wound healing, and the preparation method is as follows:

[0064] (1) Dissolve sodium polyphosphate (Na-polyP) in ultrapure water, add 20g 0.15wt% sodium polyphosphate aqueous solution to 50g mass fraction of 1.0wt% collagen aqueous solution, stir evenly, adjust pH with 0.1M NaOH is 9.5.

[0065] (2) 3g hyaluronic acid is dissolved in the ZnCl that 30mL mass fraction is 0.1wt% 2 In the aqueous solution, it is miscible with 70mL ethanol / acetone (1:1v / v) mixture, and fully stirred.

[0066] (3) The collagen-containing solution was injected into the hyaluronic acid-containing solution at a speed of 5 μL / s by using a micro-injector, and stirred for 18 hours to form a uniform emulsion. Add carbodiimide solution for cross-linking for 2h (content: 0.3%).

[0067] (4) The hydrogel microspheres were collected by centrifugation, and the hydrogel microspheres were washed 6 times with ultrapure water to remov...

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Abstract

The invention relates to injectable artificial dermis for promoting wound healing as well as a preparation method and application of the injectable artificial dermis. The injectable artificial dermis for promoting wound healing comprises collagen-polysaccharide composite hydrogel microspheres loaded with polyphosphate. The microstructure of the hydrogel microsphere is composed of a cross-linked polymer network, the hydrogel microsphere has high permeability, internal and external circulation of nutrient substances and substance exchange of metabolites are facilitated, and meanwhile, the hydrogel microsphere serves as an injectable stent to support cell ingrowth and induce cell proliferation and differentiation; due to the small size, the materials can be injected to a specific part, and the high viscosity after injection can be guaranteed; and aiming at a wound surface with irregular depth and a cavity, the dressing has good effects of filling and covering and promoting repair of tissues in a lacuna. Meanwhile, the loaded amorphous polyphosphate is hydrolyzed under the action of alkaline phosphatase, chemical energy is released, energy needed for wound healing is provided, cell proliferation, growth and migration are promoted, and formation of a vascular network is accelerated.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering regeneration, and relates to a bioactive wound repair material and its preparation method and application, in particular to an injectable artificial dermis for promoting wound healing and its preparation method and application. Background technique [0002] Artificial dermis is a tissue engineered dermis substitute, which is suitable for the repair and reconstruction of dermal layer defects. After implanted in the dermal defect, the artificial dermis acts as a template for the dermal scaffold, and fibroblasts and vascular endothelial cells grow into the collagen sponge scaffold from the wound base and surrounding tissues, and become vascularized within 2-3 weeks; after complete vascularization, the collagen sponge scaffold Gradually degrade and be replaced by new dermal tissue, so as to complete the wound repair and restore its appearance and function. [0003] CN108853590A discloses a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/26A61L27/20A61L27/58
CPCA61L27/60A61L27/58A61L27/26A61L27/20A61L2400/06A61L2300/112A61L2300/604A61L2300/412C08L89/00C08L85/02
Inventor 毛诗哲王丹妍尹家瑞梁嘉美谭荣伟许孟强郭远军刘曦李瑞佘振定
Owner SHENZHEN QIKANG MEDICAL DEVICES
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