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Multi-wavelength fingerprint spectrum-based prismatomeris glabra root quality evaluation method

A fingerprint and quality evaluation technology, applied in the field of quality evaluation based on multi-wavelength fingerprints, can solve the problems of insufficient spectral information and small differences, and achieve the effects of comprehensive information, improved accuracy, and high accuracy of results.

Pending Publication Date: 2022-04-08
ZHEJIANG UNIV OF TECH
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects and deficiencies that the spectrum information detected by a single wavelength is not comprehensive enough and the difference is small, and provide a method for evaluating the quality of yellow root in mountains based on multi-wavelength fingerprints

Method used

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  • Multi-wavelength fingerprint spectrum-based prismatomeris glabra root quality evaluation method
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  • Multi-wavelength fingerprint spectrum-based prismatomeris glabra root quality evaluation method

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Embodiment 1

[0041] What this embodiment provides is the extraction process of the yellow root decoction pieces in the mountains, specifically according to the following steps:

[0042] (1) Preparation of the mountain yellow root sample:

[0043]Take about 100g of Shanli Huanggen decoction pieces, use a decoction pot device, add 7 to 9 times of water to fully soak for 30 minutes, heat to boil, decoct for 30-60 minutes, pass through a 200-mesh sieve while hot, and obtain the medicinal residue and the first Shanli Huanggen Extract liquid; add 5 to 7 times water to the dregs, heat to boil, decoct for 20-40 minutes, filter while hot, and combine the two liquid medicines. The sampling of the extraction process was repeated for 19 batches from different origins, and a total of 19 combined extracts were obtained. Take 50 mL of 19 combined extracts and freeze-dry them. Finally, put 0.1 g of the freeze-dried powder in a 5 mL volumetric flask, dilute to 5 mL with methanol, and sonicate for 30 min. ...

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Abstract

The invention discloses a multi-wavelength fingerprint spectrum-based prismatomeris glabra root quality evaluation method, and belongs to the technical field of traditional Chinese medicine quality control. The method comprises the following steps: firstly, carrying out multi-wavelength detection and data acquisition on the prismatomeris glabra root decoction pieces by utilizing an HPLC-DAD technology to obtain a multi-wavelength fusion atlas corresponding to the prismatomeris glabra root decoction pieces, so that the fingerprint atlas of the prismatomeris glabra roots becomes a rod-shaped virtual atlas capable of reflecting rich information of each wavelength at the same time; according to the method, the advantages of comprehensiveness and integrity of multi-wavelength information are reflected through multivariate statistical analysis such as traditional Chinese medicine fingerprint spectrum similarity evaluation, hierarchical clustering analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA); the limitation that the overall quality of the medicinal materials is difficult to comprehensively evaluate when a single wavelength or a single component is used as quality control is avoided.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine quality control, and relates to a quality evaluation method based on multi-wavelength fingerprints. Background technique [0002] Fingerprint of traditional Chinese medicine is currently the most widely used identification method that can reflect the uniformity and stability of the internal quality of traditional Chinese medicine. It can meet the technical requirements of practicability, specificity and reproducibility of quality control of traditional Chinese medicine. It is an effective means to control the quality of traditional Chinese medicine and natural medicine. However, the existing fingerprints of traditional Chinese medicines are basically detected at a single wavelength. However, the components of traditional Chinese medicines are complex, and different chemical components respond differently under different ultraviolet wavelengths. How to scientifically and ration...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
Inventor 张慧白芮姜慧洁颜继忠
Owner ZHEJIANG UNIV OF TECH
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