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Preparation method of isoflavone and saponin in soybeans

A technology of isoflavones and soybeans, applied in the field of medicine, can solve the problems of high cost, low content of active substances, meager profits, etc., and achieve the effect of improving enterprise profits and improving utilization rate.

Inactive Publication Date: 2022-04-12
山东东宇生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, due to the low content of active substances such as soybean isoflavones and soybean saponins in soybeans, extraction and separation are difficult, and the technology and enterprises for extracting active ingredients currently use soybeans as raw materials to extract a single medicinal ingredient. Minimal corporate profits extracted

Method used

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  • Preparation method of isoflavone and saponin in soybeans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0016] Add 300 kg of defatted soybean germ to 2400 L of methanol: ethyl acetate = 1:1 mixed solvent, then boil and reflux for 2 hours, repeat twice, combine the extracts to obtain liquid A containing soybean isoflavones, and heat the extracted soybean materials to recover the solvent , then add 2400L of 90% methanol to reflux and extract twice, combine the extracts to obtain liquid B containing soybean saponin, finally heat the extracted soybean material to recover the solvent and then dry it to obtain soybeans for feed, and concentrate liquids A and B separately Recover the solvent to obtain aqueous solutions C and D, put the solution C containing soybean isoflavones at rest for 24 hours, add water, centrifuge, and dry to obtain soybean isoflavones with a content of 65%, add hydrochloric acid to the solution D containing soybean saponins to adjust the pH to 3, and then let it stand for 24 hours Dried after centrifugation to obtain soybean saponin with a content of 72%.

Embodiment approach 2

[0018] Add 300 kg of defatted soybean germ to 2400 L of mixed solvent of methanol: ethyl acetate = 2:3, then boil and reflux for 2 hours, repeat twice, combine the extracts to obtain liquid A containing soybean isoflavones, and heat the extracted soybean materials to recover the solvent , then add 2400L of 90% methanol to reflux and extract twice, combine the extracts to obtain liquid B containing soybean saponin, finally heat the extracted soybean material to recover the solvent and then dry it to obtain soybeans for feed, and concentrate liquids A and B separately Recover the solvent to obtain aqueous solutions C and D, put the solution C containing soybean isoflavones for 24 hours, add water, centrifuge, and dry to obtain soybean isoflavones with a content of 75%, add hydrochloric acid to the solution D containing soybean saponins to adjust the pH to 3, and then let it stand for 24 hours Dried after centrifugation to obtain soybean saponin with a content of 78%.

Embodiment approach 3

[0020] Add 300 kg of defatted soybean germ to 2400 L of ethanol: ethyl acetate = 1:1 mixed solvent, then boil and reflux for 2 hours, repeat twice, combine the extracts to obtain liquid A containing soybean isoflavones, and heat the extracted soybean materials to recover the solvent , then add 2400L of 90% ethanol to reflux for extraction twice, combine the extracts to obtain liquid B containing soybean saponin, finally heat the extracted soybean material to recover the solvent and then dry it to obtain soybeans for feed, and concentrate liquids A and B separately Recover the solvent to obtain aqueous solutions C and D, put the solution C containing soybean isoflavones at rest for 24 hours, add water, centrifuge, and dry to obtain soybean isoflavones with a content of 82%, add hydrochloric acid to the solution D containing soybean saponins to adjust the pH to 3, and then let it stand for 24 hours Dried after centrifugation to obtain soybean saponin with a content of 76%.

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Abstract

The invention discloses a preparation method of isoflavone and saponin in soybean, which belongs to the technical field of medicine, and comprises the following steps: heating, refluxing and extracting the soybean twice by using a mixed solution of ethyl acetate and one of methanol or ethanol or acetone as an extraction solvent, merging the extracting solutions, concentrating to obtain a concentrated solution 1, standing the concentrated solution 1 overnight, and concentrating to obtain a concentrated solution 2; adding water, heating and dispersing again, cooling overnight, centrifugally separating, drying the cake to obtain high-content soy isoflavone, drying the extraction residue, adding high-concentration methanol or ethanol, extracting twice, recovering the solvent from the extracting solution under reduced pressure, adding acid to adjust the pH value to 3-4, standing overnight, centrifugally separating, and drying the cake to obtain soy saponin. According to the technology, the utilization rate of the soybeans is improved in a mode of extracting and separating a plurality of products step by step, so that the enterprise profit is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of isoflavones and saponins in soybean. Background technique [0002] Soybean is rich in functional ingredients, especially the unique isoflavones and saponins as the main functional ingredients, which have health care functions such as anti-cancer and anti-aging. Soy isoflavones in soybeans is a general term for a class of isoflavones, generally including daidzin, daidzin, genistin and their aglycones, a total of 6 components, and soybean saponin is a more complex mixture of saponins. In recent years, people have paid more and more attention to soybeans. Soybean products have also become one of the most respected health foods in the world today. Because soy isoflavones have significant estrogen-like effects, soy isoflavones are also known as "plant-based natural estrogens". Active substance extraction and separation technology and medicinal p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K131/00
CPCA61K36/48A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 缪世伟李民升
Owner 山东东宇生态科技有限公司