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Method for cleaning spotting head of chip spotting instrument

A technology of a spotting head and a spotting instrument, which is applied in the directions of cleaning methods, cleaning methods and utensils, chemical instruments and methods using liquids, etc. The effect of shortening time

Pending Publication Date: 2022-04-12
HUZHOU HEALTHDIGIT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a cleaning method for the spotting head of a chip spotting instrument with short cleaning time, clean cleaning, and no corrosion of the spotting head, so as to solve the problems of spotting instruments such as HD2003. Difficult to clean the sample head, easy to cause blockage, more time for cleaning and easy to damage the sample head, etc.

Method used

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  • Method for cleaning spotting head of chip spotting instrument
  • Method for cleaning spotting head of chip spotting instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for cleaning the sampling head of a chip spotting instrument of the present invention is carried out according to the following steps:

[0052] (1) Prepare two reagents in advance:

[0053] A: 3% (V / V) Hellmanex III aqueous solution (a cleaning agent produced by HELLMA Analytics, Germany) and 5% (W / V) SDS aqueous solution,

[0054] B: 75% (V / V) isopropanol aqueous solution.

[0055] (2), add 300ul of A solution in the No. 1 hole and the No. 3 hole of the sample strip, and add 300ul of the B solution in the No. 2 hole and the No. 4 hole;

[0056] The cleaning process is:

[0057] (1) Wash the inner wall of the sampling head with purified water 10-15 times (250ul each time), and the outer wall 2 times (2 seconds each time),

[0058] (2) The sampling head absorbs 80ul of liquid A in the No. 1 hole of the sample loading strip, and soaks the sampling head in the remaining liquid A in the No. 1 hole for more than 10 minutes;

[0059](3) Wash the inner wall of the...

Embodiment 2

[0067] A method for cleaning the sampling head of a chip spotting instrument of the present invention is carried out according to the following steps:

[0068] (1) Prepare two reagents in advance:

[0069] A: 2% (V / V) Hellmanex III aqueous solution (a cleaning agent produced by HELLMA Analytics, Germany) and 10% (W / V) SDS aqueous solution,

[0070] B: 80% (V / V) isopropanol aqueous solution.

[0071] (2), add 300ul of A solution in the No. 1 hole and the No. 3 hole of the sample strip, and add 300ul of the B solution in the No. 2 hole and the No. 4 hole;

[0072] The cleaning process is:

[0073] (1) Wash the inner wall of the sampling head with purified water 10-15 times (250ul each time), and the outer wall 2 times (2 seconds each time),

[0074] (2) The sampling head absorbs 80ul of liquid A in the No. 1 hole of the sample loading strip, and soaks the sampling head in the remaining liquid A in the No. 1 hole for more than 10 minutes;

[0075] (3) Wash the inner wall of t...

Embodiment 3

[0083] A method for cleaning the sampling head of a chip spotting instrument of the present invention is carried out according to the following steps:

[0084] (1) Prepare two reagents in advance:

[0085] A: 4% (V / V) Hellmanex III aqueous solution (a cleaning agent produced by HELLMA Analytics, Germany) and 1% (W / V) SDS aqueous solution,

[0086] B: 70% (V / V) isopropanol aqueous solution.

[0087] (2), add 300ul of A solution in the No. 1 hole and the No. 3 hole of the sample strip, and add 300ul of the B solution in the No. 2 hole and the No. 4 hole;

[0088] The cleaning process is:

[0089] (1) Wash the inner wall of the sampling head with purified water 10-15 times (250ul each time), and the outer wall 2 times (2 seconds each time),

[0090] (2) The sampling head absorbs 80ul of liquid A in the No. 1 hole of the sample loading strip, and soaks the sampling head in the remaining liquid A in the No. 1 hole for more than 10 minutes;

[0091] (3) Wash the inner wall of th...

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Abstract

The invention discloses a spotting head cleaning method of a chip spotting instrument. The method comprises the following steps: preparing a reagent: preparing a solution A, wherein the solution A comprises 2-4% of a Hellmanex III aqueous solution and 1-10% of an SDS (Sodium Dodecyl Sulfate) aqueous solution; the solution B is a 70-80% isopropyl alcohol water solution; adding the solution A into the holes 1 and 3 of the sample adding plate strip, and adding the solution B into the holes 2 and 4 of the sample adding plate strip; cleaning: cleaning inner and outer walls with purified water; absorbing the liquid A in the first hole, and soaking the liquid A in the residual liquid A in the first hole; cleaning inner and outer walls with purified water; absorbing the liquid B in the second hole, and soaking the liquid B in the residual liquid B in the second hole; cleaning inner and outer walls with purified water; absorbing the solution A in the hole 3, and soaking the solution A in the residual solution A in the hole 3; cleaning inner and outer walls with purified water; absorbing the liquid B in the hole 4, and soaking the liquid B in the residual liquid B in the hole 4; cleaning inner and outer walls with purified water. The cleaning method can solve the problems that the spotting head is difficult to clean, easy to block, long in time consumption, easy to damage and the like.

Description

technical field [0001] The invention belongs to the technical field of biological instruments and relates to a method for cleaning a sampling head of a chip spotting instrument. Background technique [0002] The chip spotter is the main equipment for preparing biochips by contact method. The spotting method of the chip spotting instrument can be divided into two types: non-contact spray spot and contact spot spot. Contact chip spotting instrument: the spotting needle (pitting head) is directly in contact with the surface of the solid phase support, and the DNA sample is left on the solid phase support. The HD2003 spotting instrument produced by Mingyuan Shukang has a spotting head made of glass and metal, and the middle cavity is narrow and small. During the sampling process, the absorbed protein / nucleic acid / pigment and other substances are easily adsorbed on the inner surface of the sampling head cavity, causing the sampling head to be blocked, and the liquid droplets ca...

Claims

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Application Information

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IPC IPC(8): B08B3/08B08B3/04C11D1/14C11D3/06
Inventor 朱汝祥谢露刘荣欢
Owner HUZHOU HEALTHDIGIT CO LTD
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