Microelectrode for continuous electrochemical detection of bioactive substances, biosensor and preparation method

A technology of biologically active substances and microelectrodes, applied in sensors, scientific instruments, applications, etc., can solve problems such as obstacles to ion transmission, affecting signal stability and sensitivity, and increasing the complexity of operations

Pending Publication Date: 2022-04-12
嘉善君圆新材料科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing portable dynamic blood glucose sensors generally contain 2 to 3 electrodes. During use, the front ends of all the electrodes need to be implanted under the human skin and stay on the superficial layer of the human skin, so at least 2 to 3 wounds are generated, which not only increases the The complexity of the operation is increased, and more woun

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microelectrode for continuous electrochemical detection of bioactive substances, biosensor and preparation method
  • Microelectrode for continuous electrochemical detection of bioactive substances, biosensor and preparation method
  • Microelectrode for continuous electrochemical detection of bioactive substances, biosensor and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The present embodiment provides a kind of microelectrode, and its preparation method is as follows:

[0083] (1) Prepare the inner core of magnetic material alloy wire coated with silver and silver chloride on the surface

[0084] Nickel wire is used as the core body, with a diameter of 0.04 mm, and the surface adopts magnetron sputtering or electrochemical or chemical silver plating. This embodiment specifically uses magnetron sputtering silver plating, and then hydrochloric acid or chloride solution can be used to generate silver chloride. In this embodiment, potassium chloride solution is specifically used to generate silver chloride. The electrode is immersed in 30mmol / L KCl solution for chlorination, the ring-shaped platinum sheet is used as the reference-counter electrode, and the constant voltage chronopotentiometric method is adopted, and the cathode current is fixed at 0.5mA / cm 2 , after chlorination for an appropriate time, wash and dry with deionized water. ...

Embodiment 2

[0102] This embodiment provides a microelectrode, which is made of a brass alloy and has a hollow needle tube with an inner diameter of 0.1 mm and an outer diameter of 0.25 mm. Insert the hollow needle tube into 0.5mol / L sulfuric acid solution, take it out after a certain period of time, wash and dry it for use. The pore size of the prepared porous nanostructure is about 100nm, the structure is complete, and the pore size distribution is uniform. The results are shown in image 3 .

[0103] Other can refer to embodiment 1.

Embodiment 3

[0105] This embodiment provides a microelectrode, the outer tube of the porous metal material is filled with a heat-cured polymer electrolyte stock solution.

[0106] A chemically cross-linked gel polymer electrolyte was prepared by thermal polymerization in situ electrolyte gel method. The monomer, cross-linking agent, initiator and pure liquid electrolyte are formulated into a precursor mixture in a certain composition, and then injected into the outer tube of metal material, sealed, and then heated at a certain temperature and time to polymerize to form a gel . The preferred composition is: 20% water-soluble monomer, 30% oligomer, 2% initiator, 20% Al 2 o 3 Sol, 10% cellulose nanocrystals, the rest is potassium chloride solution. The preferred heating temperature is 70° C. and the heating time is 2 hours to form a hydrogel structure.

[0107] The inner core of the magnetic material alloy wire plated with silver and silver chloride is inserted into the outer tube by micr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Outer diameteraaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a microelectrode for continuous electrochemical detection of bioactive substances, a biosensor and a preparation method, and relates to the field of electrochemical sensors. One end of the microelectrode for continuous electrochemical detection of bioactive substances is a connecting end, the other end of the microelectrode is an insertion end, and the microelectrode comprises an outer tube and an inner core which are respectively used as two electrodes connected with an external circuit. The outer tube comprises a porous tube body made of a metal material, noble metal films or carbon films deposited on the inner and outer surfaces of the porous tube body, carbon nanotubes fixed inside and outside the porous structure, and an enzyme reaction reagent; the inner core comprises a core body made of a magnetic material, and a silver and silver chloride film plated on the surface of the core body; the same end of the outer pipe is sleeved outside the inner core, and polymer electrolyte is filled between the outer pipe and the inner core. The positive electrode and the negative electrode are combined into a whole, the number of wounds on the human body is reduced, and stability and sensitivity of monitoring signals are guaranteed.

Description

technical field [0001] The application relates to the field of electrochemical sensors, in particular, to a microelectrode for continuous electrochemical detection of biologically active substances, a biosensor and a preparation method. Background technique [0002] With the development of society, the advancement of science and technology, and the improvement of people's living standards, health issues have become the focus of many people's daily attention, and more and more quick detection products have been produced to facilitate people to quickly and easily monitor their own health status. learn. Among them, biosensors are used for continuous detection of biologically active substances in the body, and play an important role in health monitoring, disease diagnosis and treatment. The monitoring of blood sugar is very important for diabetic patients, and the blood sugar value helps to evaluate the glucose metabolism disorder of diabetic patients. Real-time continuous dyn...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N27/327A61B5/1486
Inventor 宗小林邬奎吉文婷吴春生陈大刚刘福生周敏
Owner 嘉善君圆新材料科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap