ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide

A technology of targeting peptides and mixtures, applied in the field of biomedicine, can solve the problems of large molecular weight, high cost and unstable structure of monoclonal antibodies, and achieve the effect of inhibiting the infection of new coronavirus

Active Publication Date: 2022-04-19
ZHEJIANG UNIV
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular weight of monoclonal antibodies is large, the blocking efficiency is low, the structure is unstable, and it is easy to lose activity at room temperature.
Therefore, the production, transportation and storage of monoclonal antibodies all require low temperature conditions, and the cost is high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide
  • ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide
  • ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Using the phage 12 peptide library constructed by the phage display technology, four rounds of screening were conducted for ACE2 protein-specific binding positive polypeptides. Due to the large abundance of the phage 12 peptide library, 10 μL of the phage 12 peptide library was taken for screening during the screening process, and the experiment successfully obtained the polypeptide sequence that can efficiently target the ACE2 protein through the following operation steps.

[0042] 1.1. Resuscitation and culture of host bacteria E.coil.ER2738

[0043] Prepare LB solid-state culture plates and place in a 37 °C incubator for 1 h. Using a bacterium pick stick, dip the melted E.coil.ER2738 bacterial solution, apply a "Z" shape to the surface of the LB plate, and place in a 37 °C incubator for 12 hours. Add 5 mL of liquid LB medium containing tetracycline to the shaker tube, pick out the monoclonal colonies in the plate using a sterile tip and place the tip in the medium. Place...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an ACE2 targeting peptide in targeting combination with ACE2 protein and application of the ACE2 targeting peptide. The ACE2 targeting peptide is a polypeptide, and the amino acid sequences of the ACE2 targeting peptide are respectively as follows: FPHWHGNHKHNR, GFKSHFHHNHMR, AHYHRHPPHLNR, LSPHFHHHRGAK, METRPVAPHEFR, LHRPHFHAHNNS, GHQGHWYGMFRA, YPWHHRHHMPVHP, SHKHHWPYHAH and FHGHGSHNKHR; the bioactive substance is formed by forming ACE2 targeting peptide on the surface of a mixture of microorganisms and polymers. The ACE2 protein can be efficiently targeted, the combination of the novel coronavirus spike protein and the ACE2 protein is blocked, and the effect of inhibiting novel coronavirus infection is further achieved.

Description

Technical field [0001] The present invention belongs to a polypeptide in the biomedical field and its uses, in particular to a polypeptide capable of targeting binding angiotensin-converting enzyme 2 (ACE2) and its uses. Background [0002] Angiotensin-converting enzyme 2 (ACE2) is a protein present on the surface of a variety of cells. The spike protein on the surface of the new coronavirus can target the recognition of the ACE2 protein, and through the interaction of the spike protein with ACE2, the new coronavirus can enter the human cells, causing pneumonia. [0003] Usually, researchers mainly use monoclonal antibodies to block the binding of the ACE2 protein to the new coronavirus spike protein. However, monoclonal antibodies have large molecular weight, low blocking efficiency, unstable structure, and are prone to inactivity at room temperature. Therefore, the production, transportation and preservation of monoclonal antibodies require low temperature conditions and high ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/14
CPCC07K7/08A61P31/14A61K38/00Y02A50/30
Inventor 毛传斌鲍青杨涛杨明英
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap