Animal cell tissue culture device and culture method

Abstract

The invention relates to the technical field of animal cell tissue culture, in particular to an animal cell tissue culture device and a culture method.The device comprises a culture platform, a connecting column, a culture frame, a cover plate, a shaking mechanism, a bearing disc, a culture dish and a feeding mechanism; a rotating disc drives a vertical moving block to move up and down on an annular adjusting plate in a reciprocating manner, so that the vertical moving block synchronously drives a bearing disc to shake in the process of driving the bearing disc to rotate through a shaking rod, and a nutrient solution in a culture dish is shaken and shaken up; in the feeding mechanism, according to the amount of nutrient solutions needed in all culture dishes, a valve is opened, air is extruded through an injection cylinder, the nutrient solutions are pumped into the culture dishes through cooperation of a liquid distribution pipe and a connecting pipe, a cover plate is not opened in the whole feeding process, and therefore the possibility that infectious microbes enter a cavity is reduced, and the feeding efficiency is improved. And the possibility of animal tissue cell culture polluted by infectious microbes is reduced.

Description

technical field [0001] The invention relates to the technical field of animal cell tissue culture, in particular to an animal cell tissue culture device and a culture method. Background technique [0002] Animal cell culture is a method of taking out relevant tissues from the animal body, placing the tissues in the culture medium to allow these tissue cells to grow and proliferate to obtain new cells. With the development of genetic engineering and other cell engineering technologies, cell culture technology has become It has become the basis of transgenic technology, biopharmaceuticals and many other technologies, and plays an important role in modern biotechnology. [0003] Manually place the scraped animal tissue cells in the middle of the culture dish with a scraper. Since the animal tissue cells need to consume the nutrients in the culture solution during the process of proliferation and growth, the concentration of nutrients in the culture solution will automatically c...

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Problems solved by technology

[0003] Manually place the scraped animal tissue cells in the middle of the culture dish with a scraper. Since the animal tissue cells need to consume the nutrients in the culture solution during the process of proliferation and growth, the concentration of nutrients in the culture solution will automatically change during the culture of animal tissue cells. The middle part of the petri dish gradually decreases towards the periphery of the petri dish, and there is a problem of uneven nutrient composition in the culture medium, which affects the proliferation effect of animal tissue cells

[0004] Since animal tissue cells may need to replenish the culture medium in time during the culture process, the traditional culture medium replenishment process needs to manually open the culture device and directly add the culture medium to the culture dish. This process may cause airborne bacteria to enter the culture medium. In the dish, bacteria enter the culture solution and affect the growth of animal tissue cells, and even harmful bacteria may cause the proliferation of animal tissue cells to stop, affecting the results of animal tissue cell culture

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