Novel resistance genes associated with disease resistance in soybean
A gene, soybean technology, applied in the field of novel resistance genes associated with disease resistance in soybean, can solve problems such as yield loss
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[0173] The following examples are not intended to be an exhaustive catalog of all the different ways in which the invention may be practiced or of all the features that may be incorporated into the invention. Those skilled in the art will appreciate that numerous changes and additions can be made to the different embodiments without departing from the invention. Therefore, the following description is intended to illustrate some specific embodiments of the present invention, and not exhaustively describe all permutations, combinations and changes thereof.
example 1
[0174] Example 1: Identification of ASR Resistant Glycine Glycine Lines
[0175] Multiple wild Glycine max (Glycine sojae, Penghu soybean, Glycine sojae) lines were evaluated for ASR resistance against sixteen rust strains collected in various environments. Individual pustule-derived isolates from USDA-ARS (FL Q09, FL Q12, LABR13, FLQ11) and field populations (FL Q15, NC06, Vero, GLC15, UBL, BR south and BR central )) Individual pustule-derived isolates generate rust data. Screening takes place in a closed facility.
[0176]Each wild Glycine line was evaluated over the course of multiple days of infection and graded at different time points. Using a large and diverse panel of rust isolates, each entry was screened >2 times with approximately 4 plants each in North and South America.
[0177] Wild Glycine lines were screened for ASR resistance and the following lines showed broad resistance to all ASR strains tested: PI440935, PI483193, PI595799, PI339656 and PI509501.
example 2
[0178] Example 2 : Allele mining and association to PI440935, PI483193, PI595799, PI339656, or PI509501ASR locus
[0179] The resistant parental line was crossed with a susceptible line of the same species (ie Glycine max x Glycine max and Glycine penghu x Glycine penghu) and F1 plants were generated (see Table 5). F1 plants were self-fertilized and F2 seeds were harvested from selfed F1 plants. About 200 F2 seeds were sown, and the leaf tissue of each plant was collected for DNA preparation, and then the plants were inoculated with Phakopsora pachyrhizi to determine the resistant / susceptible phenotype of each F2 individual. Tissues from 50 resistant F2 and 50 susceptible F2 were combined in separate pools and genomic DNA was prepared from each pool. For each of these pools, Illumina sequencing libraries were prepared from DNA and each library was sequenced in two Illumina HiSeq2000 2x100 bp paired-end (PE) lanes. The average yield per sample was 383 million read pairs...
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