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Lactic acid bacteria capable of degrading biogenic amine and application thereof

A technology of lactic acid bacteria and biogenic amines, applied in the field of microorganisms, can solve problems such as difficulty in ensuring food safety

Pending Publication Date: 2022-04-26
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are certain limitations on the degradation of biogenic amines in food, and it is difficult to ensure food safety

Method used

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  • Lactic acid bacteria capable of degrading biogenic amine and application thereof
  • Lactic acid bacteria capable of degrading biogenic amine and application thereof
  • Lactic acid bacteria capable of degrading biogenic amine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Screening of strains

[0035] 1. Primary screening

[0036] Weigh 10g of distiller's yeast in 100mL of sterile saline, shake and cultivate in a shaker incubator at 37°C for 30min. Pipette the suspension and dilute to 10 -6 , draw 100 μL of appropriate gradient dilutions and spread them evenly on the MRS solid medium, culture at 37°C for 32-48 hours, select single colonies of different shapes for streaking, isolation, purification and storage.

[0037] The purified strain was inoculated in MRS liquid medium for activation, and the concentration of the bacterial solution was adjusted to 1×10 8 CFU / mL, add 1mL of biogenic amine (chromogenic) medium to a 96-well sterile plate in advance, transfer 100 μL of the bacterial solution to a 96-well sterile plate, culture at 37°C for 48 hours, and use no culture medium as blank Control, record the experimental results, observe the color change of the medium, such as figure 1 As shown, the purple (A) or red (B) is positive (a...

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Abstract

The invention belongs to the technical field of microorganisms, and relates to a lactic acid bacterium capable of degrading biogenic amine and application of the lactic acid bacterium, the lactic acid bacterium is NXU-Q12, the preservation number of the lactic acid bacterium is CGMCC No.23704, and the preservation date is November 01, 2021. The screened lactic acid bacteria NXU-Q12 can degrade various biogenic amines, also has certain acid-resistant and ethanol-resistant capabilities, and can be used for preparing grape wine and Chinese wolfberry fruit vinegar.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a lactic acid bacterium capable of degrading biogenic amines and an application thereof. Background technique [0002] Biogenic amines (BA for short) are a class of low-molecular-weight nitrogen-containing organic compounds widely present in fermented foods. Amino acid decarboxylases mainly produced by microorganisms act on the decarboxylation of amino acids to form BAs. According to their structures, BAs can be divided into three categories: heterocyclic family, aromatic and aliphatic. Biogenic amines are ubiquitous in fermented foods. Moderate amounts of biogenic amines contribute to the normal physiological activities of the human body. Excessive intake of biogenic amines can cause the expansion of arteries and microvessels, leading to adverse physiological reactions such as diarrhea, headache, abdominal cramps, and vomiting. It can even lead to death. At present, the d...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12G1/022C12J1/04A23L5/20C12R1/25C12R1/02
CPCC12G1/0203C12J1/04A23L5/28C12G2200/05
Inventor 田晓菊徐佳敏张惠玲
Owner NINGXIA UNIVERSITY
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