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Application of miRNA molecular marker in schizophrenia detection

A molecular marker, schizophrenia technology, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., to achieve the effect of highlighting substantive characteristics

Pending Publication Date: 2022-04-29
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of miRNA molecules in biomedical detection of schizophrenia is still in its infancy, and its application requires new and more effective solutions in order to fully play its role

Method used

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  • Application of miRNA molecular marker in schizophrenia detection
  • Application of miRNA molecular marker in schizophrenia detection
  • Application of miRNA molecular marker in schizophrenia detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Acquisition and functional annotation of differentially expressed genes;

[0041] To identify the differentially expressed genes between schizophrenia patients and healthy individuals, the previously reported RNA-Seq data (GEO accession number GSE121376) was obtained. Use the locally developed Perl script to delete the low-quality bases at the 5' and 3' ends (Q20 ≤20). The read fragment was aligned to the human reference genome (GRCm38.p5) using HISAT 2 (version 2.0.5). BEDTools was used for reading and counting (Quinlan 2014), and RPKM (Reading Per Million Bases) method was used to calculate its expression level. The differentially expressed genes were screened by using the multiple change threshold of |log2FC|>0.4, and the P value was obtained by T-test. Subsequently, DAVID 6.8 was used for functional enrichment analysis of schizophrenia-related genes.

Embodiment 2

[0042] Example 2: Construction of ceRNA network:

[0043] Collect miRNA from miRbase. The mRNA and lncRNA sequences were obtained from NCBI(GRCm38.p5). All differentially expressed mRNA and lncRNA were used for target identification. The regulatory relationship between miRNA-mRNA and miRNA-lncRNA was determined by RNA in situ hybridization (set the coordinates of seed region to 1 to 6, 2 to 7, 3 to 8, 4 to 9 and 5 to 10; Mfe≤-25kcal / mol, p≤0.01) and BLASTN(E value is 1000, except miRNA and miRNA in forward alignment (alignment position is greater than 6), and the intersection point is obtained. Use Cytoscape(3.5.1) to visualize ceRNA network.

Embodiment 3

[0044] Example 3: Establishment of Schizophrenia Mouse Model:

[0045] The N- methyl -D- aspartic acid (NMDA) glutamate receptor antagonist MK-801(Sigma-Aldrich, St.Louis, MO., USA) was dissolved in physiological saline and injected into mice by intraperitoneal injection for 7 days (0.6mg / kg once a day). Mice under the same conditions were injected with the same dose of normal saline as control. PET and qPCR experiments were performed within 1 week after injection.

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Abstract

The invention discloses application of a miRNA molecular marker in schizophrenia detection, which is characterized in that the whole genome difference of gene expression between a schizophrenia patient and a healthy control group is analyzed by using a bioinformatics method, and then signal pathways and functions in which differential genes participate are analyzed through GO and KEGG function enrichment; then establishing a ceRNA network to analyze the interaction between the upstream key miRNA and the central target gene RASD2 and predict the optimal binding sites of the upstream key miRNA and the central target gene RASD2; the expression of DEGs is adjusted through miRNA, and hsa-miRNA-4763-3p of a targeted RASD2 gene is used as a potential biomarker and a treatment target of schizophrenia. The miR-4763-3p disclosed by the invention can be used as a potential biomarker and a treatment target of the schizophrenia, and a theoretical basis is provided for further clinical research of the schizophrenia.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a molecular marker of schizophrenia, miRNA-4763-3p, and its application in the fields of screening of high-risk groups of schizophrenia, early diagnosis and treatment of schizophrenia, etc. technical background [0002] Schizophrenia is a common severe mental illness with obstacles in perception, thinking, emotion and behavior and uncoordinated mental activities. It affects more than 20 million people around the world, with a high incidence in adolescence or early adulthood. The early mortality rate of patients is 2-3 times higher than that of ordinary people. Its typical clinical symptoms are hallucinations and delusions. In the early stage of the disease, patients are withdrawn and unwilling to communicate with others. With the further development of the disease, they are manifested as disordered thinking, hallucinations and auditory hallucinations, and some patients may even...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/178
Inventor 赵春华王娇黄琳史宏伟齐雯歆刘翠萍张诗博
Owner SHANGHAI UNIV
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