Preparation method of ganoderma lucidum selenium-enriched burdock root fermented tea
A fermented tea and selenium-enriched technology, applied in the field of fermented food, to enhance immunity, regulate blood pressure, blood sugar and blood lipids, and improve indigestion
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Embodiment 1
[0031] A kind of preparation method of ganoderma lucidum selenium-enriched burdock root fermented tea of the present invention, its steps are as follows:
[0032] 1) Preparation of seed liquid: Inoculate the ganoderma strains from the slant culture medium or glycerol storage tubes into the MRS medium, shake and culture in a shaker at 37°C and 180r / min for 28-36h;
[0033] 2) Preparation of selenium-enriched fermentation medium: adding the selenium-enriched solution sterilized by ultraviolet rays into sterile water, then adding burdock root powder after mechanical wall breaking, and mixing evenly;
[0034] 3) Preparation of selenium-enriched fermentation liquid: inoculate the seed liquid into the selenium-enriched fermentation medium in step b at a ratio of 12% (v / v), place in a shaking table at 32° C. for 48-60 h;
[0035] 4) Collect mycelial medium: culture the fermented selenium-enriched fermentation broth for mycelia, place it in a light-proof environment, grow at a tempe...
Embodiment 2
[0043] A kind of preparation method of ganoderma lucidum selenium-enriched burdock root fermented tea of the present invention, its steps are as follows:
[0044] 1) Preparation of seed liquid: Inoculate the ganoderma strains from the slant culture medium or glycerol storage tubes into the MRS medium, shake and culture in a shaker at 37°C and 180r / min for 28-36h;
[0045] 2) Preparation of selenium-enriched fermentation medium: adding the selenium-enriched solution sterilized by ultraviolet rays into sterile water, then adding burdock root powder after mechanical wall breaking, and mixing evenly;
[0046] 3) Preparation of selenium-enriched fermentation liquid: inoculate the seed liquid into the selenium-enriched fermentation medium in step b at a ratio of 15% (v / v), place in a shaker at 32-37° C. for 48-60 h;
[0047] 4) Collect mycelial medium: culture the fermented selenium-enriched fermentation broth for mycelia, place it in a light-proof environment, grow at a temperature...
Embodiment 3
[0055] A kind of preparation method of ganoderma lucidum selenium-enriched burdock root fermented tea of the present invention, its steps are as follows:
[0056] 1) Preparation of seed liquid: Inoculate the ganoderma strains from the slant culture medium or glycerol storage tubes into the MRS medium, shake and culture in a shaker at 37°C and 180r / min for 28-36h;
[0057] 2) Preparation of selenium-enriched fermentation medium: adding the selenium-enriched solution sterilized by ultraviolet rays into sterile water, then adding burdock root powder after mechanical wall breaking, and mixing evenly;
[0058] 3) Preparation of selenium-enriched fermentation liquid: inoculate the seed liquid into the selenium-enriched fermentation medium in step b at a ratio of 18% (v / v), place in a shaker at 35° C. for 48-60 h;
[0059] 4) Collect mycelial medium: culture the fermented selenium-enriched fermentation broth for mycelia, place it in a light-proof environment, grow at a temperature ...
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