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Structural variation molecular marker related to goat hypoxia adaptation and application

A technology of structural variation and molecular markers, applied in the field of genetic biology, can solve problems such as the lack of molecular markers, achieve the effects of accurate and reliable methods, promote the breeding process, and simple operation

Active Publication Date: 2022-05-06
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A problem with the [0005] prior art is the lack of a molecular marker associated with hypoxic adaptation in goats

Method used

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  • Structural variation molecular marker related to goat hypoxia adaptation and application
  • Structural variation molecular marker related to goat hypoxia adaptation and application
  • Structural variation molecular marker related to goat hypoxia adaptation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Genome-wide analysis of different genotypes and hypoxic adaptability of goats

[0035] For 86 East Asian high-altitude goats (14 Nima goats, 24 Cuoqin goats, 10 Nyingchi goats, 11 Ritu goats, 9 Shannan goats, 5 Naqu goats and 13 other goats) and 80 East Asian low-altitude goats (29 Inner Mongolia white cashmere goats, 15 Chengdu Ma goats, 9 Jintang black goats, 5 Liaoning cashmere goats, 5 Leizhou goats, 5 Matou goats and 12 other goats) Whole-genome scanning analysis found that the insertion or deletion structural variation molecular marker located at base 42098934 of goat chromosome 26 (the goat reference genome is GCF_001704415.1) was most closely related to the hypoxic adaptation of goats (Fisher test P <0.01).

[0036] A large number of studies on the correlation between genome variation and hypoxia adaptation were conducted on goats from different regions and breeds, and a significant correlation was found with goat hypoxia adaptation (P=2.76×10 -11 ),...

Embodiment 2

[0037] Example 2. Identification of Goat PAPSS2 Gene Structural Variation Polymorphism

[0038] 1. Extract the genomic DNA of the goat to be tested

[0039]The blood samples of 29 goats living in plateau areas and 44 goats living in non-plateau areas were collected from various places in China. The details are shown in Table 1. The genome in the blood was extracted using the blood genome DNA column mini-extraction kit (CW2087M). DNA.

[0040] Table 1 Sample Collection Form

[0041] sample Sample size (only) Altitude Types of Tibet Shannan Dobuzhang 10 3700m plateau Shannan Tibetan sheep 10 >3000m plateau Tibetan sheep in Banma County 9 1800–4000m plateau Kashgar cashmere goat 10 500–1200m non plateau Xinjiang Sawafuqi goat 10 1100m non plateau Hulunbeier cashmere goat 14 1000–1200m non plateau Xinjiang Bogda cashmere goat 10 450–800m non plateau

[0042] Note: Goats living in plateaus have low...

Embodiment 3

[0056] Example 3. The polymorphism site of the PAPSS2 gene of 216 goats was analyzed by expanding population

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Abstract

The invention belongs to the technical field of genetic biology, and particularly relates to a structural variation molecular marker related to goat hypoxia adaptation and application. The structural variation molecular marker is located at 245bp insertion / deletion at the 42098934th basic group of the 26th chromosome of a goat, particularly insertion / deletion at the 98bp of a nucleotide sequence as shown in SEQ ID No. 1, and the nucleotide sequence of insertion / deletion is as shown in SEQ ID No. 2. The invention also provides a specific primer pair and a detection method for detecting the molecular marker with the structural variation. The sequence of the specific primer pair is shown as SEQ ID No.3 and SEQ ID No.4. The invention also provides a detection method for detecting the molecular marker with the structural variation. The molecular marker with the structural variation is not limited by factors such as age and sex of goats, and can be used for introducing goats with high altitude hypoxia adaptability and breeding varieties.

Description

technical field [0001] The invention belongs to the technical field of genetic biology, and in particular relates to a structural variation molecular marker related to goat hypoxic adaptation and its application. Background technique [0002] Goats are considered to be one of the first domesticated animals, dating back to about 10,000 years ago, and gradually spread to various parts of the world with the migration of humans. To adapt to the extreme environmental conditions of the plateau, goats have developed unique physiological and genetic characteristics. The plateau environment refers to the plateau area with an altitude greater than 2500m, which is challenging for both local residents and domesticated animals. Andeans, Ethiopians, Tibetans, and their domestic animals are the main settlers of high-altitude environments, developing unique physiological and genetic adaptations to overcome low oxygen and high ultraviolet radiation. Studying the regulatory mechanism of suc...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125C12Q2535/101
Inventor 王小龙吴玉江李超陈炳春蔡蓓
Owner NORTHWEST A & F UNIV
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