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A molecular marker, identification primer set, kit and application for identifying fruit mulberry cultivar Yuelinda 10

A molecular marker and detection kit technology, applied in the identification of variety resources and germplasm innovation, can solve the problems affecting the validity and authority of new variety identification, large environmental impact, and long test cycle, and achieve reliable and intuitive results. High detection efficiency and the effect of overcoming uncertainty

Active Publication Date: 2022-07-29
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional identification of new mulberry varieties is mainly based on phenotypic traits, which is greatly affected by the environment, poor stability, and long testing cycle, which seriously affects the effectiveness and authority of new variety identification

Method used

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  • A molecular marker, identification primer set, kit and application for identifying fruit mulberry cultivar Yuelinda 10
  • A molecular marker, identification primer set, kit and application for identifying fruit mulberry cultivar Yuelinda 10
  • A molecular marker, identification primer set, kit and application for identifying fruit mulberry cultivar Yuelinda 10

Examples

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Embodiment 1

[0034] Example 1 Screening and obtaining of specific primer sequences for a new fruit mulberry variety 'Guangdongda 10'

[0035] 1. SSR primer selection

[0036] The MISA software was used to search the genome sequence in the mulberry genome database, and the SSR sites in the genome sequence were retrieved. The length of the searched SSR motif repeating unit was 2 to 6 nucleotides, and 2, 3, and 4 were set respectively. The minimum search repetitions of 5, 6 nucleotides are 6, 5, 5, 4, and 4, and the length of the flanking sequence of the SSR site is ≥150 bp. According to the searched SSR site, use Primer3v2.3.4 ( http: / / primer3.sourcego-rge.net) software to design SSR primers. The design criteria are: primer length is 18-25bp, annealing temperature is 57-63°C, GC content is 40-70%, and the length of PCR product is 100 -300bp, randomly select SSR sites that can be compared with the NCBI non-redundant protein database to synthesize 300 pairs of primers, each pair of primers co...

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Abstract

The invention discloses a molecular marker, identification primer set, kit and application for identifying fruit mulberry cultivar 'Yuetianda 10'. The present invention utilizes the fluorescent SSR technology to screen 300 pairs of SSR primers designed and developed for the mulberry genome sequence, and confirms that the core primers marked M8179 and M10360 can respectively amplify two specific mulberry varieties 'Yuetianda 10'. A new mulberry cultivar 'Yuelingda 10' can be identified from Guangdong mulberry germplasm and popularized varieties and strains, which can be used for rapid identification and detection of a new mulberry cultivar 'Yuelingda 10'. The present invention identifies the 'Yueyu Da 10' by directly detecting the M8179 marker and / or the M10360 marker, which overcomes the uncertainty of its identification due to external morphological features, has simple operation, high detection efficiency, reliable and intuitive results, and is fruitful. The protection of the new mulberry variety 'Yuelingda 10' provides scientific and technical support, and the invention is beneficial to the popularization, utilization and protection of the variety.

Description

technical field [0001] The invention relates to the technical field of identification of variety resources and germplasm innovation, in particular to a molecular marker, identification primer set, kit and application for identification of a fruit mulberry variety 'Guangdongda 10'. Background technique [0002] Mulberry is a kind of fruit resource with excellent characteristics and is the representative of the third generation fruit. It has been listed by the Ministry of Health of the People's Republic of China as "both food and medicine". In recent years, with the improvement of people's living standards and health awareness As a new fruit variety, mulberry is increasingly favored by people because of its unique taste, rich nutrition and high active substances, and has gradually become more and more popular. Into the families of ordinary people, they are accepted and loved by the citizens. In addition to fresh food, mulberries are processed and developed into mulberry juice...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11C12Q1/686
CPCC12Q1/6895C12Q1/686C12Q2600/156C12Q2525/151C12Q2563/107
Inventor 王振江罗国庆唐翠明陈莲钟建武林森
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI