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High-pressure environment deep-sea microorganism enrichment and multi-level purification device and method

A high-pressure environment and purification device technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problem of inability to improve the success rate of microbial enrichment culture and obtain high-purity microbial populations and other issues to achieve the effect of improving the success rate of enrichment culture

Active Publication Date: 2022-05-10
GUANGDONG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the anaerobic microbial high-pressure culture device provided by this solution has the advantages of convenient liquid extraction under high-pressure environment and less slag in the extracted liquid, it cannot improve the success rate of microbial enrichment and culture, and cannot obtain high-purity microorganisms population

Method used

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  • High-pressure environment deep-sea microorganism enrichment and multi-level purification device and method
  • High-pressure environment deep-sea microorganism enrichment and multi-level purification device and method
  • High-pressure environment deep-sea microorganism enrichment and multi-level purification device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Such as figure 1 , figure 2 , image 3 As shown, a high-pressure environment deep-sea microbial enrichment and multi-level purification device includes a data acquisition and control system 1, and the enrichment and multi-level purification device is composed of a plurality of high-pressure microbial enrichment culture kettles 2 connected in series; the high-pressure microbial The enrichment culture kettle 2 is provided with a detachable sealing cover 21 and a connecting sampling valve group, which is provided with a sensor group 22, and the detachable sealing cover 21 is designed to facilitate the insertion of culture substrates and sterilization operations; each high-pressure microbial enrichment The collection culture kettle 2 is all placed in the high and low temperature water bath container 23; wherein: the detachable sealing cover 21 is used to facilitate the sterilization operation and put into the culture substrate inside the high-pressure microorganism enrich...

Embodiment 2

[0063] More specifically, on the basis of Example 1, such as Figure 4 As shown, a high-pressure environment deep-sea microbial enrichment and multi-level purification method is realized by using a high-pressure deep-sea microbial enrichment and multi-level purification device. First, multiple high-pressure microbial enrichment culture kettles 2 are numbered. The first The high-pressure microorganism enrichment culture kettle 2 is used as the enrichment culture chamber, and the other high-pressure microorganism enrichment culture kettles 2 are divided into No. 1 separation and purification culture chamber and No. 2 separation and purification culture chamber according to the connection position; and then specifically perform the following steps:

[0064] S1: Fill the enrichment culture chamber with the substrate to be cultured and pour the pre-packaged sterile liquid medium into each separation culture chamber;

[0065] S2: Fill the enrichment culture chamber with the culture ...

Embodiment 3

[0077] In order to fully illustrate the realization process and technical effect of this scheme, on the basis of Examples 1 and 2, this example provides a high-pressure environment enrichment, purification and cultivation device and method. Such as figure 1 , figure 2 As shown, an enrichment culture device with the same high-pressure and low-temperature environmental conditions as the in-situ environmental conditions of deep-sea methanophilic bacteria is constructed, and it has the operation method of enhancing mass transfer, and can sample and analyze in real time, and detect the changes of built-in environmental parameters, which is convenient for carrying out Adjusting the cultivation process is the key to the realization of this example. Such as figure 1 As indicated, the high-pressure microorganism enrichment culture kettle 2 involved in this example mainly includes a detachable sealing cover 21 and its accessories. The body of the high-pressure microorganism enrichm...

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Abstract

The invention provides a high-pressure environment deep-sea microorganism enrichment and multi-level purification device which is composed of a plurality of high-pressure microorganism enrichment culture kettles connected in series. A detachable sealing cover and a connection sampling valve group are arranged on the high-pressure microorganism enrichment culture kettle, and a sensor group is arranged in the high-pressure microorganism enrichment culture kettle; each high-pressure microorganism enrichment culture kettle is arranged in a high-low temperature water bath container; the invention also provides a high-pressure environment deep-sea microorganism enrichment and multi-level purification method, which is realized by applying the high-pressure environment deep-sea microorganism enrichment and multi-level purification device, and can realize enrichment culture of microorganisms living under extreme conditions, such as deep-sea barophilic bacteria, under in-situ living pressure and temperature conditions. The enrichment culture success rate of deep-sea microorganisms is effectively improved; meanwhile, deep-sea microbial populations with relatively high purity can be obtained through multi-level enrichment and dilution purification culture processes, and an important basic technical means is provided for development of deep-sea engineering bacteria.

Description

technical field [0001] The invention relates to the technical field of marine microorganisms, in particular to a high-pressure environment deep-sea microorganism enrichment and multi-level purification device and method. Background technique [0002] Microorganisms are the most abundant organisms in the ocean. Due to the uniqueness of the environment, deep-sea extremophiles often produce biomacromolecules (extremophiles) and small molecules with unique activities, which are very different from terrestrial bioactive substances in structure and function. [0003] Barophilic microorganisms are an important group in deep-sea ecosystems, and some strict barophilic bacteria cannot grow in normal pressure environments. The study of deep-sea barophilic microorganisms will help to elucidate the mechanism by which microorganisms adapt to high-pressure environments. At the same time, in the process of adapting to the environment, deep-sea microorganisms have evolved unique metabolic ...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/34C12M1/26C12M1/02C12M1/04C12M1/38C12M1/12C12M1/00C12N1/20C12N1/02
CPCC12M23/38C12M27/02C12M29/06C12M33/00C12M23/58C12M41/18C12M41/40C12M23/52C12M39/00C12M41/48C12M47/12C12M47/04C12M37/00C12N1/20C12N1/02
Inventor 冯景春张偲杨志峰
Owner GUANGDONG UNIV OF TECH
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