Multi-omics analysis method, system and equipment for direct RNA sequencing and storage medium

Abstract

The embodiment of the invention provides a multi-omics analysis method, system and equipment for direct RNA sequencing and a storage medium, and relates to the technical field of biological information processing. The method comprises the following steps: acquiring sequencing data of direct RNA sequencing; comparing the sequencing data with a reference genome to obtain sequencing comparison data; performing full-length transcript identification according to the sequencing comparison data to obtain full-length transcript sequence data; performing transcript quantitative processing on the sequencing data based on the full-length transcript sequence data to obtain transcript quantitative data; processing the sequencing data according to the methylation modification prediction model to obtain methylation modification data; processing the full-length transcript sequence data according to a newborn mRNA prediction model to obtain newborn mRNA data; and performing correlation analysis according to the full-length transcript sequence data, the transcript quantitative data, the methylation modification data and the newborn mRNA data to obtain direct RNA sequencing multi-dimensional information. The method can achieve the technical effect of high sequencing precision.

Description

technical field [0001] The present application relates to the technical field of biological information processing, in particular, to a multi-omics analysis method, system, equipment and storage medium for direct RNA sequencing. Background technique [0002] At present, in the third-generation sequencing technology, nanopore sequencing technology can directly sequence RNA molecules, also known as direct RNA sequencing technology; because the sequencing results obtained by direct RNA sequencing technology contain multiple dimensions of RNA molecules, but there is currently no A method that can mine the results of direct RNA sequencing, therefore, the realization of multi-dimensional information acquisition of the results of direct RNA sequencing has important practical significance. [0003] In the prior art, in the analysis process of RNA molecular data of the third generation sequencing technology, there are mainly the following technologies: one is to combine the second ge...

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Problems solved by technology

[0002] At present, in the third-generation sequencing technology, nanopore sequencing technology can directly sequence RNA molecules, also known as direct RNA sequencing technology; because the sequencing results obtained by direct RNA sequencing technology contain multiple dimensions of RNA molecules, but there is currently no A method that can mine the results of direct RNA sequencing, so it has important practical significance to achieve multi-dimensional information acquisition of direct RNA sequencing results

[0003] In the prior art, in the analysis process of RNA molecular data of the third generation sequencing technology, there are mainly the following technologies: one is to combine the second generation sequencing technology for quantitative analysis of full-length transcripts, which generally uses the second generation sequencing technology The obtained RNA sequence is combined with the RNA sequence measured by the third-generation sequencing technology for quantitative analysis. The disadvantage is that the sample needs to be subjected to a second-generation sequencing in the quantitative analysis, which requires a large amount of samples and is expensive; the second is the identification and analysis of full-length transcripts. This analysis uses a qualitative method to only identify the full-length structural sequence of RNA molecules in the third-generation sequencing results. The disadvantage is that it cannot effectively use other dimensional data of RNA direct sequencing; the third is the methylation modification identification of RNA molecules. This analysis adopts the established The model predicts the methylation site of the RNA molecule, but the disadvantage is that the prediction accuracy needs to be improved, and it cannot be determined which position the methylation site is located in the original RNA molecule

There are few analysis solutions for direct RNA sequencing technology at home and abroad, all of which only analyze one or two dimensional information of RNA molecules, and the third-generation sequencing technology is expensive, and does not make full use of the results of direct RNA sequencing

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