Sample pretreatment method for multi-omics analysis, and application thereof
A sample preparation and sample technology, applied in the field of sample preparation for multi-omics analysis, can solve the problems of high transportation and storage costs, high pollution, differences, etc., and achieve good reproducibility, high sensitivity, and high accuracy. sexual effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0090] A sample pretreatment method for multi-omics analysis, specifically comprising the following steps:
[0091] (1) Preparation and sample loading of the sample pretreatment device:
[0092] The structural schematic diagram of the sample pretreatment device is as follows: figure 1 As shown, among them, 1 is the tip of the pipette, 2 is the solid phase extraction membrane, 3 is the C18 resin, and 4 is the ion exchange resin; the preparation method is as follows: 3 pieces of C18 membranes are sequentially loaded into the 200 μL pipette tip (U.S. 3M Empore), 0.5mg C18 resin particles (particle diameter 20 μm, Dr.Maisch GmbH, Germany), 2 mg ion exchange resin particles (the mass ratio of SCX / SAX is a mixed ion exchange resin of 1:1, and the particle diameter is 20 μm , U.S. AppliedBiosystems), to obtain the sample pretreatment device;
[0093] Use 80 μL of methanol and 40 μL of 10 mM ammonium bicarbonate solution (pH=7.4) to wash, activate and equilibrate the materials fille...
Embodiment 2
[0107] The difference between this example and Example 1 is that the filling amount of ion exchange resin particles in the sample pretreatment device is 1 mg; other components of the sample pretreatment device, sample pretreatment method, mass spectrometry detection method and mass spectrometry data analysis method All the same as in Example 1.
Embodiment 3
[0109] The difference between this example and Example 1 is that the filling amount of ion exchange resin particles in the sample pretreatment device is 3 mg; other components of the sample pretreatment device, sample pretreatment method, mass spectrometry detection method and mass spectrometry data analysis method All the same as in Example 1.
PUM
Property | Measurement | Unit |
---|---|---|
length | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com