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Detection kit for phthisis mitochondria-derived small RNA (Ribonucleic Acid)

A technology for detecting kits and mitochondria, applied in the field of molecular biomedicine, can solve problems such as unknown structures and functions, and achieve high stability

Pending Publication Date: 2022-05-27
LIUZHOU WORKERS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on mitochondria-derived microRNA is very limited, and its structure and function are unknown

Method used

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  • Detection kit for phthisis mitochondria-derived small RNA (Ribonucleic Acid)
  • Detection kit for phthisis mitochondria-derived small RNA (Ribonucleic Acid)
  • Detection kit for phthisis mitochondria-derived small RNA (Ribonucleic Acid)

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Embodiment Construction

[0078] In order to make the above objects, features and advantages of the present invention more clearly understood, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present invention. Therefore, the present invention is not limited by the specific implementation disclosed below.

[0079] The steps to build the model are as follows:

[0080] (1) Blood small RNA extraction and sequencing: Total RNA was extracted from peripheral blood mononuclear cells using TRIzol reagent (Invitrogen) and purified using mirVana miRNA isolation kit (Ambion, Thermo Fishe...

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Abstract

The invention discloses a pulmonary tuberculosis mitochondria-derived small RNA (Ribonucleic Acid) detection kit. The pulmonary tuberculosis mitochondria-derived small RNA detection kit is characterized by comprising a pair of reverse transcription primers and detection primers corresponding to up-regulation of the expression ratio of mitochondria-derived small RNA and 6 pairs of down-regulation of the expression ratio of mitochondria-derived small RNA. The invention provides a specific expression profile of a mitochondrial-derived small RNA (mdsRNA) ratio in peripheral blood of a pulmonary tuberculosis patient, and a reverse transcription primer, a detection primer, a detection kit and a diagnosis model of the specific expression profile, and provides support for early discovery and early treatment of clinical pulmonary tuberculosis.

Description

【Technical field】 [0001] The invention belongs to the technical field of molecular biomedicine, and particularly relates to a detection kit for mitochondrial-derived small RNA of pulmonary tuberculosis. 【Background technique】 [0002] Tuberculosis is a chronic pulmonary infectious disease caused by Mycobacterium tuberculosis and is the second largest infectious disease in the world. Current diagnostic tools include smear microscopy, microbial cultures, and molecular testing with M. tuberculosis detection kits. Smear microscopy provides rapid results and is widely used in clinical laboratories, but this traditional method has a low positivity rate of only 20 to 30 percent. Microbial culture examinations take longer (4-8 weeks). In the early stages of TB infection, an unresolved challenge in TB diagnosis is accurately distinguishing other lung diseases from TB with similar clinical symptoms and radiological features. Mycobacterium tuberculosis detection kit (CN101935693B). ...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6883C12N15/11
CPCC12Q1/689C12Q1/6883C12Q2600/158C12Q2600/178Y02A50/30
Inventor 凌宙贵胡灼君韦晓谋唐贞明温中薇汤滢其他发明人请求不公开姓名
Owner LIUZHOU WORKERS HOSPITAL
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