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Method for producing hyaline cartilage tissue in vitro

A technology of cartilage tissue and cartilage, applied in the field of in vitro production of hyaline cartilage tissue, can solve the problems of expanding and differentiating cells and restricting the use of difficult elderly patients

Pending Publication Date: 2022-05-27
瓦纳里克斯股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, another challenge of chondrocyte-based cell therapy is the difficulty in expanding and differentiating cells from elderly patients, which limits their use in this population

Method used

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  • Method for producing hyaline cartilage tissue in vitro
  • Method for producing hyaline cartilage tissue in vitro
  • Method for producing hyaline cartilage tissue in vitro

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0159] Materials and Methods

[0160] Chemicals

[0161] XAV939 was obtained from Sigma and used at a concentration of 10 μM in medium E for 4 days in 2D culture.

[0162] Production of Cartibeads

[0163] The following media described in Table 1 were used in the examples.

[0164] Table 1: Composition of the media used in the examples

[0165]

[0166]

[0167] sample collection

[0168] Human cartilage samples were obtained from lifeconsenting donors (ages 18 to 80 years) following orthopaedic surgery for various indications (Table 2).

[0169] Table 2: Donor characteristics, including gender, age, and pathology.

[0170]

[0171]

[0172] The collected cartilage was transferred to the laboratory in sterile receivers containing normal saline (NaCl 0.9%) at room temperature. Human cartilage sample collection has been approved by the Swiss Ethics Committee (BASEC, 2016-00656).

[0173] The cartilage of the minipigs was obtained from a lateral trochlearhold...

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Abstract

The present invention relates to a novel method for producing cartilage tissue in vitro, as well as therapeutic uses and screening methods using the cartilage tissue produced thereby.

Description

technical field [0001] The present invention relates to a novel method of producing hyaline cartilage tissue ("Cartibead") in vitro, as well as therapeutic uses and screening methods using the cartilage tissue thus produced. Background technique [0002] Hyaline cartilage is composed of specialized cells called chondrocytes and a surrounding extracellular matrix. The matrix is ​​synthesized and secreted by chondrocytes and is mainly composed of type II collagen fibers, glycosaminoglycans (GAGs) and 60-80% water. The hyaline cartilage has four layers above the subchondral bone: a superficial layer, an intermediate layer, a deep layer, and a calcified layer. The biomechanical properties of articular cartilage depend to a large extent on the composition and integrity of the extracellular matrix. [0003] Cartilage has a very limited ability to repair itself, and once injured, it usually develops into osteoarthritis (OA). Aging and repetitive trauma, such as occurs during hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077A61L27/36A61L27/38
CPCC12N5/0655A61L27/3817A61L27/3895A61L27/3852A61L27/3683A61L27/3612A61L27/3654C12N2501/115C12N2501/15C12N2501/415C12N2506/13C12N2513/00A61L2430/06C12N2500/02
Inventor V·蒂昂
Owner 瓦纳里克斯股份公司