Chenopodium quinoa willd CqALS gene mutant, molecular identification method and application

Abstract

The invention discloses a chenopodium quinoa CqALS gene mutant, a molecular identification method and application, the chenopodium quinoa CqALS2-1 gene mutant is chenopodium quinoa CqALS-5, G at the 1609th site of the chenopodium quinoa CqALS2-1 gene is mutated into T, and the chenopodium quinoa CqALS gene mutant generates resistance to chemical drug (ASL) herbicides. The nucleotide sequence of the CqALS2-1 is as shown in SEQ ID No. 1 (sequence identifier number 1). After the gene of the CqALS gene mutant CqALS-5 of the quinoa is mutated, the herbicide resistance of the quinoa is obviously improved, a good model is provided for the large-scale production of the quinoa, the production cost can be saved by using a herbicide, meanwhile, the use of labor force is reduced, and a certain foundation is laid for the large-scale production of the quinoa.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a quinoa CqALS gene mutant and a molecular identification method and application. Background technique [0002] Quinoa (Chenopodium Quinoa Willd), belonging to the family Chenopodium Quinoa Willd, is a dicotyledonous, tetraploid, annual herb. The United Nations Food and Agriculture Organization (FAO) named 2013 the International Year of Quinoa to promote quinoa to the world. In 2020, more than 100 countries around the world are conducting planting trials or commercial production of quinoa, but one of the biggest problems encountered in quinoa production is the many weeds associated with the growth of quinoa, such as gray rape, rape, etc. The herbicides that have not yet been registered can be removed by difficult to find suitable methods. Domestically (in China), the problem of weeds is mainly solved by manual weeding. As a result, the labor cost per hectare of producti...

AI Technical Summary

Problems solved by technology

In 2020, more than 100 countries around the world are doing planting experiments or commercial production of quinoa, but one of the biggest problems encountered in quinoa production is the many weeds associated with the growth of quinoa, such as gray vegetables, rapeseed, etc. Unregistered herbicides can be used to find a suitable way to remove them. In China (in China), the problem of weeds is mainly solved by manual weeding. This results in an increase of 45 million yuan in labor costs per hectare of production costs ( Based on the production cost in 2021), in addition to the cost increase, there is often a risk of labor shortage in the weeding season in concentrated quinoa production areas, and weed treatment in quinoa scale production has become an important limiting factor, and the production cost increase, and the consumer side has an impact

Most of the herbicide ingredients currently on the market are ALS inhibitors, which destroy the synthesis of valine, leucine and isoleucine in plants by inhibiting ALS, but the use of ALS inhibitors is not only effective for weeds, but also for Quinoa also has a killing effect

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