Construction method of multi-channel-chip type self-energized sensor for high-throughput detection of porcine diarrhea coronavirus

A construction method and coronavirus technology, applied in the field of electrochemical biosensing, can solve the problem of multi-channel-chip self-powered sensors not being developed, achieve good Fermi level matching, excellent detection performance, promote miniaturization and integrated effect

Pending Publication Date: 2022-06-03
湖南圣测生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, research on multi-channel-on-a-chip self-powered sensors based on spatially resolved strategies has not been carried out.

Method used

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  • Construction method of multi-channel-chip type self-energized sensor for high-throughput detection of porcine diarrhea coronavirus
  • Construction method of multi-channel-chip type self-energized sensor for high-throughput detection of porcine diarrhea coronavirus
  • Construction method of multi-channel-chip type self-energized sensor for high-throughput detection of porcine diarrhea coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) ZnIn 2 S 4 Preparation of:

[0051] Measure 0.0732g Zn(CH 3 COO) 2 ·2H 2 O and 0.1953g InCl 3 ·4H 2 O was dissolved in 10 mL (CH 2 OH) 2 , stir evenly to obtain solution A; measure 0.2 g of TAA, add it into solution A, stir evenly to obtain solution B, transfer solution B to a stainless steel autoclave for solvothermal reaction, and react at 160 ° C for 16 h, the reaction is complete After washing with deionized water and ethanol, the solid product ZnIn was obtained after drying. 2 S 4 .

[0052] (2)Cu 2 Preparation of O-CuO:

[0053] First, 1.165g (Cu(NO 3 ) 2 ·3H 2 O) be dissolved in 40mL deionized water, stir, obtain solution C; Then add 2mL ammonium hydroxide (25wt%) dropwise to solution C, obtain solution D, transfer solution D to stainless steel autoclave for hydrothermal reaction. After the completion of the reaction, washed with deionized water and ethanol, and dried to obtain a solid product Cu 2 O-CuO.

[0054] figure 2 Yes Example 1 i...

Embodiment 2

[0066] (1) ZnIn 2 S 4 Preparation of:

[0067] Measure 0.1464g Zn(CH 3 COO) 2 ·2H 2 O and 0.1953g InCl 3 ·4H 2 O was dissolved in 10 mL (CH 2 OH) 2 , stir evenly to obtain solution A; weigh 0.2 g of TAA, add it to solution A, stir evenly to obtain solution B, transfer solution B to a stainless steel high pressure reaction kettle for solvothermal reaction, and react at 140 ° C for 16 h, the reaction is complete After washing with deionized water and ethanol, the solid product ZnIn was obtained after drying. 2 S 4 .

[0068] (2)Cu 2 Preparation of O-CuO:

[0069] First, 1.7475g (Cu(NO 3 ) 2 ·3H 2 O) be dissolved in 40mL deionized water, stir, obtain solution C; Then add 2mL ammonium hydroxide (25wt%) dropwise to solution C, obtain solution D, transfer solution D to stainless steel autoclave for hydrothermal reaction. After the completion of the reaction, washed with deionized water and ethanol, and dried to obtain a solid product Cu 2 O-CuO.

[0070] Steps (3)...

Embodiment 3

[0072] (1) ZnIn 2 S 4 Preparation of:

[0073] Measure 0.0732g Zn(CH 3 COO) 2 ·2H 2 O with 0.3906g InCl 3 ·4H 2 O was dissolved in 10 mL (CH 2 OH) 2 , stir evenly to obtain solution A; measure 0.2 g of TAA, add it into solution A, stir evenly to obtain solution B, transfer solution B to a stainless steel high pressure reaction kettle for solvothermal reaction, react at 180 ° C for 16 h, the reaction is complete After washing with deionized water and ethanol, the solid product ZnIn was obtained after drying. 2 S 4 .

[0074] (2)Cu 2 Preparation of O-CuO:

[0075] First, 0.5825g (Cu(NO 3 ) 2 ·3H 2 O) be dissolved in 40mL deionized water, stir, obtain solution C; Then add 2mL ammonium hydroxide (25wt%) dropwise to solution C, obtain solution D, transfer solution D to stainless steel autoclave for hydrothermal reaction. After the completion of the reaction, washed with deionized water and ethanol, and dried to obtain a solid product Cu 2 O-CuO.

[0076] Steps (3...

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Abstract

The invention belongs to the technical field of electrochemical biosensing, and discloses a construction method of a multichannel-chip type self-energized sensor for high-throughput detection of porcine diarrhea coronavirus. The method comprises the following steps: firstly, preparing an integrated chip electrode of a ZnIn2S4 modified photoanode region and a Cu2O-CuO modified photocathode region; and then, constructing a multi-channel-chip type self-energized sensor capable of detecting the three porcine diarrhea coronaviruses. According to the invention, a laser etching technology is adopted, a photoanode and a photocathode are integrated on the same chip, and immune recognition elements of various target objects are separated according to regions based on a spatial resolution strategy, so that the recognition reaction and signal output of each target object do not interfere with each other, thereby realizing high-throughput specific detection of three viruses, namely PEDV, PDCoV and TGEV. The chip integrated electrode promotes the miniaturization and integration development of sensing equipment, and has the advantages of high-throughput detection capability, short detection time, small sample consumption and low test cost.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensing, and relates to a multi-channel-chip self-powered sensor based on a spatial resolution strategy and a construction method thereof. technical background [0002] Porcine viral diarrhea has a high incidence, wide distribution and great harm. Coronaviruses are the main pathogens that cause swine viral diarrhea, including porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and porcine delta coronavirus (PDCoV). The main clinical symptoms of sick pigs infected by the above viruses are vomiting, diarrhea, dehydration and intestinal bleeding, which increases the difficulty of etiological diagnosis. At the same time, sick pigs are often accompanied by recessive infection or mixed infection of multiple pathogens, which makes the disease more serious, infectious and fatal. Traditional swine diarrhea-like coronavirus detection methods include: path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/327G01N33/543G01N33/569
CPCG01N27/416G01N27/3278G01N33/56983G01N33/54346G01N2333/17G01N2333/165Y02A50/30
Inventor 王坤高允魏婕胡琴琴
Owner 湖南圣测生物科技有限公司
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