Method for quantitatively detecting SiO2-FO targeted nano-drug carrier on basis of direct competitionfluorescence immunoassay
A nano-drug carrier and fluorescent immunology technology, which is applied in the fields of fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of expensive instruments, cumbersome operations, low stability and sensitivity, and achieve strong specificity, Simple operation and high sensitivity effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0048] The preparation method of each solution that the present invention relates to is:
[0049] PBS solution (0.01mol / L pH=8.0): weigh 8.0g of NaCl, 0.1g of KCl, NaH 2 PO 4 2H 2 00.106g, Na 2 HPO 4 12H 2 O 3.34g was dissolved in distilled water and the volume was adjusted to 1000mL.
[0050] Carbonate buffer CB (0.5mol / L pH=9.6): weigh Na 2 CO 3 1.59g, NaHCO 3 2.94g was dissolved in distilled water and the volume was adjusted to 100mL.
[0051] PBST solution (0.01mol / L pH=8.0): add 500 μL Tween-20 to 1000 mL PBS, and mix well.
[0052] Coating buffer CB (0.05mol / L pH=9.6): weigh Na 2 CO 3 1.59g, NaHCO 3 2.94g was dissolved in distilled water and the volume was adjusted to 1000mL.
[0053] 1wt% casein solution: it is a blocking solution, weigh 0.01g of casein and dissolve it in 1mL of PBS, and mix well.
[0054] MES buffer solution (0.1M, pH=5.5): Dissolve 0.1921g MES in 10mL distilled water, adjust its pH to 5.5 with NaOH solution.
Embodiment 1
[0056] Quantitative detection of folic acid functionalized silica (SiO2) based on direct competition fluorescence immunoassay 2 -FO) a method for targeting a nano-drug carrier, the method comprising the steps of:
[0057] a. SiO 2 - Preparation of FO-coated antigens and immunogens
[0058] a-1. Weigh 10mg SiO2 2 -FO was dissolved in 1mL PBS solution, and magnetically stirred for 1h to obtain SiO with a concentration of 10mg / mL 2 -FO solution;
[0059] a-2. Weigh 10mg OVA and dissolve it in 2mL PBS, and add the SiO obtained in step a-1 while stirring 2 -FO solution, after mixing evenly, add 90 μL 25% glutaraldehyde solution dropwise, and stir for 4 hours at 25°C in the dark; put the reaction solution in a dialysis bag with a molecular weight cut-off of 8000-12000Da, dialyze it with PBS for 24 hours and collect it Get SiO 2 -FO-coated antigen with a concentration of 3.33 mg / mL, stored at 4°C until use;
[0060] a-3. Weigh 10mg BSA and dissolve it in 2mL PBS, and add the S...
PUM
Property | Measurement | Unit |
---|---|---|
molecular weight | aaaaa | aaaaa |
particle diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com