Eriomycorus cerinus repellent and application thereof
The technology of bee stink bug and solvent is applied in the application, animal repellent, biocide and other directions, which can solve the problems such as the decline of chemical control effect, and achieve the effect of good egg laying and anti-feeding and avoiding effect, environment-friendly and convenient to use.
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Embodiment 1
[0021] Example 1: Y-Tube Behavioral Response
[0022] Experiments were carried out under natural light conditions with room temperature of 24±1°C and relative air humidity of 40±5%. Before the experiment, the Y-type olfactometer (Y-type tube) was washed with acetone and n-hexane successively. Decanal, heptanal, nonanal and linalool were prepared with n-hexane into three concentration gradients of 0.1 μg / μL, 1 μg / μL and 10 μg / μL to be tested. Using n-hexane as the control, take 10 μL of the test sample. Drop the sample and n-hexane on two different filter papers respectively, put the two filter paper strips into the odor source device bottles on both sides of the Y-shaped tube, turn on the air sampler and make the airflow in the Y-shaped tube 100mL. / min, and then put the male and female adult worms into the release tube and observe for 5 min. For the first time, the odorant was considered to have selected the odor source when its arm was more than 5cm and the duration was mor...
Embodiment 2
[0024] Example 2: EAG Relative Response Values
[0025] EAG (Syntech, Germany) was used to measure the electrophysiological responses of male and female adult worms to different concentrations of decanal, heptanal, nonanal and linalool. The specific operation steps are as follows: decanal, heptanal, nonanal and linalool are prepared with n-hexane into three concentration gradients of 1 μg / μL, 10 μg / μL and 100 μg / μL. Drop 10 μL of the sample to be tested or n-hexane on the filter paper, and then put the filter paper into the sample tube. Cut off the antennae of the adult Pseudomonas spp. from the base, cut off a small part (about 1 mm) from the end of the antennae, and glue the two ends of the antennae to the fork electrodes using conductive glue. Adjust the continuous air flow at the antennae to 5mL / s. When testing, switch the continuous air flow to the stimulating airflow of the sample to be tested, that is, the continuous air flow is suspended, the stimulated airflow of th...
Embodiment 3
[0027] Example 3: Field experiment
[0028] Test site: Soybean field in the experimental field of Fuyang Academy of Agricultural Sciences, Fuyang City, Anhui Province. The planting area in this area is 40 mu.
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