Microbial herbicide and preparation method thereof
A herbicide and microbial technology, applied in the field of microbial herbicides, can solve the problems of single microbial action object and small application range, and achieve the effects of wide application range, enhanced weeding effect, and growth inhibition
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Embodiment 1
[0027] A microbial herbicide of the present invention, comprising the following components and the content of each component is 1,1'-ethyl-2,2'-bipyridine dibromohydrin 10-25%, Bacillus macrosomia 30-45%, Persimmon spores 10-25%, Helminthia grass 15-25%, Trichoderma 14-30%;
[0028] Specific to the present embodiment, 1,1'-ethyl-2,2'-bipyridine dibromo salt 10%, Bacillus macrosomia 30%, persimmonia 10%, Helminthia macrophyllum 25%, Trichoderma 25%.
[0029] The preparation method of the above microbial herbicide is as follows:
[0030] Include the following specific steps:
[0031] Step 1: Preparation medium: potato 20%, glucose 2%, yeast paste 0.5%, peptone 0.5%, the allowance is distilled water;
[0032] Step 2: After sterilizing the prepared medium, it is cultured in a sterile environment at 34 °C for 3 days;
[0033] Step 3: In a sterile environment, the cultured part of the medium is packed in 4 50ml Erlenmeyer flasks, and then the other part of the medium is evenly divided a...
Embodiment 2
[0037] A microbial herbicide of the present invention, comprising the following components and the content of each component is 1,1'-ethyl-2,2'-bipyridine dibromohydrin 10-25%, Bacillus macrosomia 30-45%, Persimmon spores 10-25%, Helminthia grass 15-25%, Trichoderma 14-30%;
[0038] Specific to the present embodiment, 1,1'-ethyl-2,2'-bipyridine dibromohydrin 20%, Bacillus macrosomia 40%, persimmonia 10%, Helminthia graminearum 15%, Trichoderma 15%.
[0039] The preparation method of the above microbial herbicide is as follows:
[0040] Include the following specific steps:
[0041] Step 1: Preparation medium: potato 20%, glucose 2%, yeast paste 0.5%, peptone 0.5%, the allowance is distilled water;
[0042] Step 2: After sterilizing the prepared medium, it is cultured in a sterile environment at 34 °C for 3 days;
[0043]Step 3: In a sterile environment, the cultured part of the medium is packed in 4 50ml Erlenmeyer flasks, and then the other part of the medium is evenly divided an...
Embodiment 3
[0047] A microbial herbicide of the present invention, comprising the following components and the content of each component is 1,1'-ethyl-2,2'-bipyridine dibromohydrin 10-25%, Bacillus macrosomia 30-45%, Persimmon spores 10-25%, Helminthia grass 15-25%, Trichoderma 14-30%;
[0048] Specific to the present embodiment, 1,1'-ethyl-2,2'-bipyridine dibromo salt 20%, Bacillus macrosomia 30%, Persimmonia 20%, Helminthia macrophyllum 10%, Trichoderma 20%.
[0049] The preparation method of the above microbial herbicide is as follows:
[0050] Include the following specific steps:
[0051]Step 1: Preparation medium: potato 20%, glucose 2%, yeast paste 0.5%, peptone 0.5%, the allowance is distilled water;
[0052] Step 2: After sterilizing the prepared medium, it is cultured in a sterile environment at 34 °C for 3 days;
[0053] Step 3: In a sterile environment, the cultured part of the medium is packed in 4 50ml Erlenmeyer flasks, and then the other part of the medium is evenly divided an...
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