Use of MBV for treatment of autoimmune diseases
An autoimmune, disease technology, applied in allergic diseases, skin diseases, bone diseases, etc.
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Embodiment 1
[0207] Example 1: Differentiated by lipid groups and RNA sequencing substrate binding puff (MBV) and extracellular bauxis (EV)
[0208] The matrix combination of nano sac balp (MBV) has been reported as part of the ECM biological stand.Although the extraordinarily the theme of the outerine of the hydraulic cells has been in -depth research, their similarity to MBV is limited to size and shape.This embodiment uses LC-MS-based lipid groups and oxidation and restore lipids to compares the liquid-phase EV and MBV phospholipids in detail.Combined with the comprehensive RNA sequencing and biological information analysis of the goods in the capsule, this embodiment indicates that MBV is a different and unique sub -group of EV, and it is the differences of bioma based on ECM.
[0209] Examples of this embodiment identify the similarity and differences between the liquid phase (ie, the external body) and the matrix binding form (ie, the MBV).However, in view of the EV that exists in biolog...
Embodiment 2
[0241] Example 2: MBV is used to treat arthritis induced by lowering alkane
[0242] Use the method described in the embodiment 1 to prepare the bladder matrix.
[0243] By buffer (50mm Tris PH7.5, 5mm CACL 2 Liberase TL (highly purified collaonase i and collaonase II) in the shake bed at room temperature at room temperature at room temperature at room temperature.The digestive ECM is then centrifuged for 30 minutes with 10,000xg to remove the ECM fragments.Then, at 4 ° C, the released MBV clarification of MBV was centrifuged at 4 ° C (Beckman Coulter Optima L-90K Ultracentrifewe) for 2 hours to precipitate MBV.
[0244] The rats that have been induced by lowering alkanes have been established as a clinical -related animal model for studying rheumatoid arthritis (TUNCEL et al. Plos One.2016; 11 (5): E0155936).On the 0th day of the study, at the 8-week-old female Sprague-Dawley rats, by injecting 300 μL of lowering alkane (2,6,10,14-four-in-four bases 15 in the tail back of the tai...
Embodiment 3
[0251] Example 3: Based on the combination of nano -saw pad to reduce synovial inflammation, joint cartilage destruction, and joint protein glycogen loss in arthritis induced by prostaglandin.
[0252] Schiladic inflammation, cartilage destruction, and protein glycotic loss are the basic tissue pathological changes that occur during the progress of the RA disease.In order to determine the impact of MBV treatment on these histological parameters, the rats are collected from an animal in the animal that 2 at the end of the study.
[0253]Each animal's hind paw is used for histological analysis.The organizational specimen is fixed with 10 % of the PBS, pH 7.4, and 5 % methyl acid deches for calcium, and buried in paraffin.Slice was stained by Su Musu and Yigong (H & E), and was used as a microscopic microscope to check arthology and pathology.Slice is dyed with tolueine blue and Irahin dyeing agent to compose protein glycogen that evaluates joint cartilage through optical microscopy....
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Abstract
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