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Application of cholesterol sulfate detection reagent in preparation of kit for auxiliary diagnosis, treatment effect monitoring and prognosis evaluation of sepsis

A technology for cholesterol sulfate and auxiliary diagnosis, which is applied in the field of cholesterol sulfate detection reagents, preparation of sepsis auxiliary diagnosis, treatment effect monitoring and prognosis evaluation kits, and can solve the problem that the role of CS is not completely clear

Active Publication Date: 2022-06-21
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007]Currently the role of CS in sepsis is not completely clear, the change of CS content in serum in the development of sepsis and its role domestically There are no reports outside

Method used

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  • Application of cholesterol sulfate detection reagent in preparation of kit for auxiliary diagnosis, treatment effect monitoring and prognosis evaluation of sepsis
  • Application of cholesterol sulfate detection reagent in preparation of kit for auxiliary diagnosis, treatment effect monitoring and prognosis evaluation of sepsis
  • Application of cholesterol sulfate detection reagent in preparation of kit for auxiliary diagnosis, treatment effect monitoring and prognosis evaluation of sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Study on the relationship between CS content in serum and sepsis

[0047] 1. Determination of CS content in serum of patients with sepsis and serum of CLP mice

[0048] 1. LC-MS / MS conditions are as follows:

[0049] (1) Chromatographic conditions

[0050] Gradient elution was used, and the chromatographic conditions were as follows:

[0051]

[0052]

[0053] Elution scheme:

[0054]

[0055] (2) Mass spectrometry conditions

[0056] Electrospray ionization source (ESI), negative ion scanning mode, GAS1: 60psi, GAS2: 60psi, Curtain GAS: 10psi, source temperature is 600℃, ion spray voltage (IS) is 4500V, declustering voltage (DP) 100V, CS The ion pair is 465.1-96.9; the collision energy is 43eV, and the ion pair of the internal standard is 265.1-223.1, and the collision energy is 45eV.

[0057] 2. Sample pretreatment

[0058] Serum samples from clinical normal healthy people and sepsis patients were collected for analysis.

[0059] The blood o...

Embodiment 2

[0082] Embodiment 2. The composition of the kit for detecting the content of CS in the serum of patients with sepsis and its use method

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Abstract

The invention provides application of a cholesterol sulfate detection reagent in preparation of a kit for auxiliary diagnosis, treatment effect monitoring and prognosis evaluation of sepsis, and belongs to the field of biomarkers. Research finds that the content of cholesterol sulfate in early serum of a sepsis patient is remarkably increased, the patient enters a shock state along with symptom aggravation, and the content of cholesterol sulfate is reduced, so that cholesterol sulfate can be used as an auxiliary index for evaluating severity of sepsis. In addition, treatment effect auxiliary monitoring and auxiliary prognosis evaluation of sepsis can be carried out according to the expression level of cholesterol sulfate in the treatment process. The kit is used for auxiliary diagnosis, auxiliary monitoring of treatment effect and auxiliary prognosis evaluation of sepsis by detecting cholesterol sulfate in serum, a method is provided for auxiliary monitoring of clinical sepsis, an effective basis is provided for taking related treatment measures or decisions for patients, and the clinical application prospect is good.

Description

technical field [0001] The invention belongs to the field of biological markers, in particular to the use of a cholesterol sulfate detection reagent in the preparation of a sepsis auxiliary diagnosis, treatment effect monitoring and prognosis evaluation kit. Background technique [0002] Sepsis is a systemic inflammatory response syndrome caused by infection that can progress to septic shock and multiple organ dysfunction syndrome, which can be life-threatening. The activation of inflammatory cells and the production of a large number of inflammatory mediators lead to multiple organ damage, dysfunction and even failure during the pathogenesis of sepsis. In recent years, with the deepening of the research on the pathogenesis and mechanism of sepsis, the concept of sepsis has been updated to sepsis 3.0, and various treatment techniques have been continuously improved, such as 1h bundle therapy, antibiotic management, immune conditioning, Organ support, etc. At present, among...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/065
Inventor 苏智广于晓倩刘银张红梅
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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