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Method for preparing mixed linear high value for centrifugal micro-fluidic chip

A microfluidic chip and centrifugal technology, which is applied in the field of medical testing, can solve the problem of not being able to evaluate multiple items at the same time, and achieve the effects of reducing testing costs, reducing matrix effects, and saving testing time

Inactive Publication Date: 2019-05-03
NINGBO MEIKANG BAOSHENG BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current linear high-value reagents are often single-item, and a group of tests cannot evaluate the linear range of multiple items at the same time, so it cannot make good use of the advantages of centrifugal microfluidic chips that can detect multiple items at the same time in one test.

Method used

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  • Method for preparing mixed linear high value for centrifugal micro-fluidic chip
  • Method for preparing mixed linear high value for centrifugal micro-fluidic chip
  • Method for preparing mixed linear high value for centrifugal micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Serum collected for physical examination, hepatitis B surface antibody, hepatitis A antibody, hepatitis C antibody, HIV antibody, etc. were all negative, and the serum was divided into two (equal volumes);

[0028] (2) Add 0.80% cholesteryl sulfate sodium salt to one of the serums, heat to 55°C, and cool to room temperature after completely dissolving;

[0029] (3) Add 0.25% urea, 0.025% creatinine, 0.035% uric acid, 0.85% glucose, 0.025% glycocholic acid, and 0.08% triolein to another serum in sequence;

[0030] (4), step (2) and step (3) gained solution are mixed;

[0031] (5), in the solution of step (4) gained, add the bovine serum albumin of 4.5%, the alanine aminotransferase of 500U / L, the aspartate aminotransferase of 500U / L;

[0032] (6) Add 5.0% mannitol and 0.1% sodium azide to the solution obtained in step (5); after fully dissolving and mixing, subpackage and freeze-dry.

[0033] The mixed linear high values ​​(sample) of the above examples were measur...

Embodiment 2

[0045] 1. Collect the serum of the health examination, the hepatitis B surface antibody, hepatitis A antibody, hepatitis C antibody, HIV antibody, etc. are all negative, and the serum is divided into two (equal volume);

[0046] 2. Add 0.9% cholesteryl sulfate sodium salt to one of the serums, heat to 60°C, and cool to room temperature after completely dissolving;

[0047] 3. Add 0.3% urea, 0.03% creatinine, 0.04% uric acid, 0.9% glucose, 0.04% glycocholic acid, and 0.09% triolein to another serum in sequence;

[0048] 4. Mix the solutions obtained in step 2 and step 3;

[0049] 5. Add 5% bovine serum albumin, 700U / L alanine aminotransferase, and 750U / L aspartate aminotransferase to the solution obtained in step 4;

[0050] 6. Add 6% Tween 80 and 0.15% PC300 to the solution obtained in step 5; after fully dissolving and mixing, subpackage and freeze-dry.

[0051] The stability of the mixed linear high values ​​of the above examples was investigated. The test was carried out...

Embodiment 3

[0053]1. Collect the serum of the health examination, the hepatitis B surface antibody, hepatitis A antibody, hepatitis C antibody, HIV antibody, etc. are all negative, and the serum is divided into two (equal volume);

[0054] 2. Add 1.0% cholesteryl sulfate sodium salt to one of the serums, heat to 65°C, and cool to room temperature after completely dissolving;

[0055] 3. Add 0.5% urea, 0.05% creatinine, 0.05% uric acid, 1.2% glucose, 0.05% glycocholic acid, and 0.12% triolein to another serum in sequence;

[0056] 4. Mix the solutions obtained in step 2 and step 3;

[0057] 5. Add 6% bovine serum albumin, 800U / L alanine aminotransferase, and 800U / L aspartate aminotransferase to the solution obtained in step 4;

[0058] 6. Add 5% sucrose, 0.5% Tween 80, and 0.2% sodium azide to the solution obtained in step 5; after fully dissolving and mixing, subpackage and freeze-dry.

[0059] The mixed linear high values ​​of the above examples are used to evaluate the linear range of...

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Abstract

Provided in the invention is a method for preparing a mixed linear high value for a centrifugal micro-fluidic chip. The method comprises: collecting healthy physical examination serum with the negative hepatitis B surface antibody, hepatitis A antibody, hepatitis C antibody, HIV antibody and the like and splitting the serum into two; adding 0.8% to 1.0% of sodium cholesteryl sulfate in one serum part, heating the mixture to reach a temperature of 55 to 65 DEG C, and carrying out cooling to reach a room temperature after complete dissolving; adding 0.2 to 0.5% of urea, 0.02 to 0.05% creatinine,0.03 to 0.05% uric acid, 0.8 to 1.2% glucose, 0.02 to 0.05% glycocholic acid, and 0. 08 to 0.12% triolein into the other serum part successively; and mixing the solutions and adding 4 to 6% bovine serum albumin, 500 to 800 U / L alanine aminotransferase, and 500 to 800 U / L aspartate aminotransferase. According to the invention, the prepared mixed linear high value is used for evaluating the linearrange performance evaluation of the centrifugal micro-fluidic chip; a technical effect of evaluating a plurality of items simultaneously by testing of one group is realized; the amount of the reagentdisks is reduced; the testing time is saved; and the testing cost is lowered.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a method for preparing a mixed linear high value of a centrifugal microfluidic chip, which can also be called a method for preparing samples for evaluating the mixed linear range of a centrifugal microfluidic chip . Background technique [0002] Reagent analysis performance evaluation is an important part of clinical laboratory quality management, which includes the linear range of reagents. The linear range is the performance of whether the final output signal of the instrument corresponding to the concentration of the analyte in the entire detection system is in a constant ratio. It is a very important performance index and an important weight to ensure the accuracy of clinical test results. [0003] The traditional linear range verification is often carried out for a single project, that is, the high concentration sample close to the upper limit of the linear range of...

Claims

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Application Information

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IPC IPC(8): G01N1/38
Inventor 邹炳德张桂春
Owner NINGBO MEIKANG BAOSHENG BIOMEDICAL ENG
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