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Indoor identification method for bolting resistance of spring Chinese cabbage

An indoor identification, spring cabbage technology, applied in the direction of instruments, testing plants/trees, measuring devices, etc., can solve the problems of time-consuming identification methods and unrepeatable identification results.

Pending Publication Date: 2022-06-21
QINGDAO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the current identification method is very time-consuming, and can only be identified once a year, and because of different natural climate conditions, the identification results are often not repeatable

Method used

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  • Indoor identification method for bolting resistance of spring Chinese cabbage
  • Indoor identification method for bolting resistance of spring Chinese cabbage
  • Indoor identification method for bolting resistance of spring Chinese cabbage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In 2020, 20 spring cabbage inbred lines (see Table 1 for specific germplasm) were accurately identified for bolting resistance indoors, and the seeds were soaked on January 16. Specifically, 24 seeds of each spring cabbage variety (breeding material) to be identified were counted. Discharge in a petri dish covered with 2 layers of wet filter paper, and each 9cm petri dish can be divided into 4 parts at intervals to hold 4 parts of the material. After the seeds are placed, add an appropriate amount of warm water with a straw, and germinate at 25°C; after the radicle stretches out on the 17th, vernalize at 4°C for 28 days (2 / 14) and 35 days (2 / 21) respectively. And 42 (2 / 28) days of sowing, 72-hole plug trays, 4 copies per tray, after sowing, cultured in tissue culture room at 24°C for 16h in light (10000LX), 20 days after sowing, the budding and flowering stages of each material were recorded daily. .

[0039] See Table 1 for vernalization at 4°C for 35 days. The ident...

Embodiment 2

[0048] Field verification test

[0049] The 20 materials involved in Implementation 1 will be raised in the greenhouse on March 6 in spring 2021, and the seedlings will be planted in the field on March 31, with a distance of 40cm between plants in two rows of 1 meter, on May 29 and June 4. The leaf bulbs were cut open to investigate the shortened stems. The investigation results are shown in Table 3.

[0050] Table 3 Results of field investigation for 20 materials

[0051]

[0052]

[0053] In the field investigation during the normal harvest period (May 29), the 20 materials were all spring cabbage materials that were relatively resistant to bolting, so the difference in the length of the shortened stem was small, and because the area of ​​the shortened stem was related to the characteristics of the material itself, so It is difficult to distinguish strong and weak bolting resistance according to the shortened stem length and shape. The survey was extended to June 4th...

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Abstract

The invention provides a method for indoor identification of bolting resistance of spring Chinese cabbages, and belongs to the technical field of Chinese cabbage cultivation. A method for indoor identification of bolting resistance of spring Chinese cabbages comprises the following steps: spring Chinese cabbage seeds are subjected to germination acceleration until radicles extend out, vernalization is performed for 34-36 days, the spring Chinese cabbages are sown into a seedling culture substrate for illumination culture, the initial flowering time of the spring Chinese cabbages is recorded, and the bolting resistance of the spring Chinese cabbages is judged according to the number of days needed from sowing to the initial flowering period; the bolting resistance is weak; in 25-29 days, the bolting resistance is medium; in 30-35 days, the bolting resistance is moderately strong; when the seeds are sown for more than 35 days and do not bloom, the bolting resistance is strong. According to the method provided by the invention, bolting resistance can be accurately identified, the operation method is simple, convenient and short in time consumption, a large number of germplasms can be simultaneously identified, and the identified spring Chinese cabbage germplasms can be applied to bolting-resistant variety breeding.

Description

technical field [0001] The invention belongs to the technical field of Chinese cabbage cultivation, in particular to a method for indoor identification of spring Chinese cabbage resistance to bolting. Background technique [0002] With the demand for the annual supply of Chinese cabbage, the winter-spring protected areas in the north and the open-field overwinter cultivation in the south have become more and more demanding on the bolting resistance of Chinese cabbage. Breeding bolting-resistant varieties requires breeding materials that are resistant to bolting, and the breeding materials are resistant to bolting. Bolting identification usually requires overwintering identification in protected areas or early sowing field identification in spring, which takes at least 4 months and more than 2 months, respectively. For example, the identification method for greenhouse overwintering Chinese cabbage is to sow seedlings from late October to early November every year, transplant ...

Claims

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Application Information

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IPC IPC(8): G01N33/02G01N33/00
CPCG01N33/025G01N33/0098
Inventor 杨晓云张清霞宋永骏司朝光张淑霞郭莹段喜政
Owner QINGDAO ACAD OF AGRI SCI
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