Method for improving anti-oxidation and immune resistance of hepialus larvae
An anti-oxidation and larval technology, applied in animal feed, animal feed, application, etc., can solve the problems of decreased survival rate of bat moth larvae, non-growth efficiency of bat moth larvae, morbidity and death of bat moth larvae, etc.
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Embodiment 1
[0011] Weigh 2 grams of Clostridium butyricum spore powder with a specification of 10 billion / gram and add it to 1000 mL of pure water, stir evenly, and obtain a concentration of 2 × 10 7 CFU / mL bacterial solution. The prepared bacterial liquid was sprayed on the soil matrix where the host larvae of Cordyceps sinensis grow, and the amount of bacteria liquid was sprayed into each cubic centimeter of the matrix. After fully stirring and mixing, 100 larvae of the same batch of hatched Cordyceps sinensis host bat moth larvae were weighed and weighed. After calculating the average single weight, it was put into the pretreated matrix, and the carrot feed was added to raise it under the environmental conditions of 8 degrees Celsius and 60% humidity. The control group was not sprayed with bacterial liquid, but was sprayed with a corresponding amount of sterile water, and other conditions were the same as the experimental group. The results of calculating the survival rate after feed...
Embodiment 2
[0013] Weigh 0.05 g of Clostridium butyricum spore powder with a specification of 10 billion / g and add it to 1000 mL of pure water, stir evenly, and obtain a concentration of 0.5 × 10 6 CFU / mL bacterial solution. The prepared bacterial liquid was sprayed on the soil matrix where the host larvae of Cordyceps sinensis grew, and the amount of bacteria liquid was sprayed into 0.01 mL per cubic centimeter of the substrate. After fully stirring and mixing, 100 larvae of the same batch of hatched Cordyceps sinensis host bat moth larvae were weighed and weighed. After calculating the average single weight, put it into the pretreated matrix, add carrot feed, and raise it under the environmental conditions of 18 degrees Celsius and 80% humidity. The control group was not sprayed with bacterial liquid, but was sprayed with a corresponding amount of sterile water, and other conditions were the same as the experimental group. The results of calculating the survival rate after feeding for...
Embodiment 3
[0015] Weigh 0.3 g of Clostridium butyricum spore powder with a specification of 10 billion / g and add it to 1000 mL of pure water, stir evenly, and obtain a concentration of 3 × 10 6 CFU / mL bacterial solution. The prepared bacterial liquid was sprayed on the soil matrix where the host larvae of Cordyceps sinensis grow, and the amount of bacteria liquid was sprayed into each cubic centimeter of the substrate. After fully stirring and mixing, 100 larvae of the same batch of hatched Cordyceps sinensis host bat moths were weighed and weighed. After calculating the average single weight, put it into the pretreated matrix, add carrot feed and raise it under the environmental conditions of 13 degrees Celsius and 75% humidity. The control group was not sprayed with bacterial liquid, but was sprayed with a corresponding amount of sterile water, and other conditions were the same as the experimental group. The results of calculating the survival rate after feeding for 120 days showed ...
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