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Application of double-antibody coupling anti-cancer drug in colorectal cancer tumor treatment

A technology for antibody coupling and anti-cancer drugs, which is applied in the direction of anti-tumor drugs, antibodies, drug combinations, etc.

Pending Publication Date: 2022-07-01
BEIJING WANHUA BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, this method has not been applied to the preparation of ADCs, but detailed methods for peptide conjugation have been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] The preparation of an immunogen

[0010] 1.1 Colorectal cancer cell sw837

[0011] Colorectal cancer cells sw837 were purchased from Shanghai Jining Industrial Co., Ltd., batch number 202009410.

[0012] 1.2 Expression and purification of endothelial growth factor (VEGF-B186)

[0013] 1.2.1 Synthesis of VEGF-B186

[0014] Check the relevant literature, select the gene sequence of endothelial growth factor (VEGF-B186), select the prokaryotic expression vector pET-32a, and send it to Suzhou Hongxun Biological Company for synthesis, that is, construct the expression vector VEGF-B186, which will be used for expression after identification protein.

[0015] 1.2.2 Expression and purification of VEGF-B186

[0016] Take the correctly identified bacterial solution and activate it in a small bottle, cultivate it overnight, and transfer it to a large bottle of shaking bacteria the next day. The growth is in logarithmic phase, and the OD 600 When it is 0.6-0.8, add IPTG with a...

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Abstract

A colorectal cancer cell sw837 and a cell endothelial growth factor VEGF-B186 are respectively used for immunizing a chicken to obtain two IgY antibodies, and the IgY (sw837) and an anti-cancer drug 5 are mixed; according to the present invention, the two IgY antibodies are subjected to coupling to obtain the antibody conjugate, the two IgY antibodies are mixed and drenched into the experimental mouse, and the test result shows that the obvious tumor inhibition effect is provided;

Description

Technical field: [0001] The invention relates to a double antibody IgY, comprising an IgY antibody conjugate, which can effectively inhibit the growth of colorectal cancer tumor cells. Background technique: [0002] Colorectal cancer is a common malignant tumor of digestive tract, and its incidence rate ranks third to fifth in the world, and its tumor-related mortality ranks fourth. In recent years, the incidence of colorectal cancer in my country has been increasing year by year. There are many monographs on colorectal cancer. Among them, Li Shirong's monograph has set up a chapter on colorectal cancer-specific immunotherapy. The relevant data of foreign scholars show that the mortality and recurrence rate of colorectal cancer treated with monoclonal antibodies are reduced by 30% to 27%. [0003] IgY antibody has the functions of acid resistance, alkali resistance and enzymatic hydrolysis resistance, and is suitable for oral treatment of gastrointestinal diseases. [0004...

Claims

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Application Information

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IPC IPC(8): A61K47/68A61K47/55A61K31/7072A61K39/395A61P35/00
CPCA61K47/6807A61K47/55A61K31/7072A61K39/39558A61K9/0053A61P35/00A61K2039/836A61K2300/00
Inventor 万积成
Owner BEIJING WANHUA BIOENG
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