Rapid thin-layer identification method for lyophilized powder of Linggui Zhugan decoction

A thin-layer identification, freeze-dried powder technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as non-thin-layer identification methods, and achieve the effects of easy reference and sample, simple method, and improved time efficiency.

Pending Publication Date: 2022-07-01
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And there is no thin-layer identification method in literature reports at presen

Method used

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  • Rapid thin-layer identification method for lyophilized powder of Linggui Zhugan decoction
  • Rapid thin-layer identification method for lyophilized powder of Linggui Zhugan decoction
  • Rapid thin-layer identification method for lyophilized powder of Linggui Zhugan decoction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of Lingguizhugan Decoction is as follows:

[0053] Lingguizhugan Decoction, 55g of Poria, 41g of Guizhi, 27g of Atractylodes Rhizoma, and 27g of Zhigancao, were extracted with water, filtered, and the medicinal liquid was collected, and freeze-dried by a vacuum freeze dryer to obtain freeze-dried powder.

[0054] Take 2 g of Linggui Zhugan Decoction freeze-dried powder, add 25 mL of a 4:1 chloroform-methanol mixture, ultrasonically treat it for 30 min, filter, evaporate to dryness, add 1 mL of methanol to the residue to dissolve, and then get the test solution; Poria cocos reference medicinal material 1 g, and the same method was used to prepare the reference medicinal material solution; separately, 2 mg of cinnamic acid and atractylodes lactone II were added, respectively, and methanol was added to make a 1 mg / mL reference solution; Atractylodes lactone Ⅱ reference solution 10μL, according to the 2015 version of the general rule 0502 thin-layer chromato...

Embodiment 2

[0056] The preparation of Lingguizhugan Decoction is as follows:

[0057] Lingguizhugan Decoction, 55g of Poria, 41g of Guizhi, 27g of Atractylodes Rhizoma, and 27g of Zhigancao, were extracted with water, filtered, and the medicinal liquid was collected, and freeze-dried by a vacuum freeze dryer to obtain freeze-dried powder.

[0058] Take 2 g of Linggui Zhugan Decoction freeze-dried powder, add 25 mL of a 4:1 chloroform-methanol mixture, ultrasonically treat it for 30 min, filter, evaporate to dryness, add 1 mL of methanol to the residue to dissolve, and then get the test solution; Poria cocos reference medicinal material 1 g, and the same method was used to prepare the reference medicinal material solution; separately, 2 mg of cinnamic acid and atractylodes lactone II were added, respectively, and methanol was added to make a 1 mg / mL reference solution; Atractylodes lactone Ⅱ reference solution 10μL, according to the 2015 version of the general rule 0502 thin-layer chromato...

Embodiment 3

[0060] exclusive inspection

[0061] Taking the test sample and reference medicinal materials described in Example 1, the reference substance as the object, and according to the same preparation method, the lack of negative control was prepared respectively. The results show that the method is applicable, such as Figure 1-Figure 3 shown.

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PUM

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Abstract

The invention discloses a rapid thin-layer identification method for lyophilized powder of Linggui curcuma zedogan decoction, which comprises the following steps: taking lyophilized powder of Linggui curcuma zedogan decoction, adding a trichloromethane-methanol mixed solution, carrying out ultrasonic treatment, filtering, drying by distillation, and adding methanol into residues to obtain a test solution; preparing a reference medicinal material solution from a poria cocos reference medicinal material by the same method; taking cinnamic acid, atractylenolide II and liquiritin reference substances, and adding methanol to prepare a reference substance solution; the reference medicinal material solution, the cinnamic acid reference substance solution and the atractylenolide II reference substance solution are sucked, and spots with the same color are shown at the positions corresponding to the cinnamic acid reference substance chromatogram and the poria cocos reference medicinal material in the test substance chromatogram; in the chromatogram of the sample to be tested, fluorescent spots with the same color are displayed under an ultraviolet lamp at the position corresponding to the chromatogram of the atractylenolide II reference substance; the method comprises the following steps: sucking a liquiritin reference substance solution and a test substance solution, and in a test substance chromatogram, displaying fluorescent spots with the same color at a position corresponding to a liquiritin reference substance chromatogram under an ultraviolet lamp.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a rapid thin-layer identification method for freeze-dried powder of Linggui Zhugan Decoction. Background technique [0002] Lingguizhugan Decoction is composed of four herbs, Poria, Guizhi, Atractylodes, and Licorice. It has the effect of warming yang and removing dampness. It is commonly used in clinical treatment of chronic bronchitis, bronchial asthma, cardiogenic edema, chronic glomerulonephritis edema, Meniere's disease, neurosis, etc. Thin-layer identification has the advantages of economy, rapidity, and multi-component index reflection. It is widely used in traditional Chinese medicine compound preparations. However, the operation of thin-layer identification of compound preparations is still cumbersome and complicated, and the identification update rate is not high. Taking the "Yiqing Granule" contained in the 2020 edition of "...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 周巧敏胡昌江姜艳娇黄宇仰莲沈东孙纪元
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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