Thin-layer chromatographic analysis method for identifying and distinguishing fermented cordyceps sinensis bacterial powder and fermented cordyceps sinensis bacterial powder

A technology of fermenting Cordyceps fungus powder and Cordyceps sinensis fungus powder, which is applied in the field of thin-layer chromatography analysis, can solve the problems of confusing sale and use, no identification and distinction, etc., and achieve the effect of increasing detection methods, no spot diffusion, and fast identification

Active Publication Date: 2022-07-08
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this invention is to solve the problem that fermented Cordyceps sinensis powder and fermented Cordyceps powder are easily confused for sale and use due to their similar appearance, and there is currently no fast and effective method for the identification and distinction of the two powders Technical problem, but provide a kind of thin-layer chromatographic analysis method for distinguishing between fermented Cordyceps sinensis powder and fermented Cordyceps powder

Method used

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  • Thin-layer chromatographic analysis method for identifying and distinguishing fermented cordyceps sinensis bacterial powder and fermented cordyceps sinensis bacterial powder
  • Thin-layer chromatographic analysis method for identifying and distinguishing fermented cordyceps sinensis bacterial powder and fermented cordyceps sinensis bacterial powder
  • Thin-layer chromatographic analysis method for identifying and distinguishing fermented cordyceps sinensis bacterial powder and fermented cordyceps sinensis bacterial powder

Examples

Experimental program
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Effect test

Embodiment 1

[0045] 1) Prepare standard control solution and sample solution

[0046] 1.1) Get the fermented cordyceps sinensis powder reference substance raw material and the fermented cordyceps fungus powder reference substance raw material, place them in a stoppered triangular flask respectively, and then add 6 times the quality of the fermented cordyceps sinensis fungus powder reference substance raw material and the fermented cordyceps sinensis powder reference substance raw material respectively The amount and mass concentration of ethanol are 70%, and ultrasonic extraction is carried out for 60 minutes, and the temperature is brought to room temperature, and then filtered with a microporous membrane, and the filtrate is collected as a standard control solution; The standard reference solution numbers of the raw materials are marked as No. 1 and No. 4, respectively.

[0047] 1.2) Take two parts of fermented Cordyceps sinensis powder and two parts of fermented Cordyceps fungus powder to...

Embodiment 2

[0062] 1) Prepare standard control solution and sample solution

[0063] 1.1) Get the fermented cordyceps sinensis powder reference substance raw material and the fermented cordyceps fungus powder reference substance raw material, place them in a stoppered triangular flask respectively, then add 5 times the quality of the fermented cordyceps sinensis powder reference substance raw material and the fermented cordyceps fungus powder reference substance raw material respectively The amount and mass concentration of ethanol were 90%, and ultrasonically extracted for 30 minutes, put it to room temperature, and then filtered with a microporous membrane, and the filtrate was collected as a standard control solution; The numbers are marked 1 and 4, respectively.

[0064] 1.2) Take two parts of fermented Cordyceps sinensis powder and two parts of fermented Cordyceps fungus powder to be identified and differentiated in the market as raw materials of bacterial powder to be tested, respec...

Embodiment 3

[0079] 1) Prepare standard control solution and sample solution

[0080] 1.1) Get the fermented Cordyceps sinensis powder reference substance raw material and the fermented cordyceps fungus powder reference substance raw material, place them in a stoppered triangular flask respectively, then add the fermented cordyceps sinensis fungus powder reference substance raw material, the fermented cordyceps sinensis powder raw material reference substance raw material quality 10 respectively. 100% ethanol, and ultrasonically extracted for 50 minutes, put it to room temperature, then filtered with a microporous membrane, and collected the filtrate as a standard control solution; the standard control of fermented Cordyceps sinensis powder and fermented cordyceps The liquid numbers are marked as No. 1 and No. 4, respectively.

[0081] 1.2) Take two parts of fermented Cordyceps sinensis powder and two parts of fermented Cordyceps fungus powder to be identified and differentiated in the mar...

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Abstract

The invention relates to a thin-layer chromatographic analysis method, in particular to a thin-layer chromatographic analysis method for identifying and distinguishing fermented cordyceps sinensis bacterial powder and fermented cordyceps sinensis bacterial powder. The method solves the technical problems that the fermented cordyceps sinensis fungus powder and the fermented cordyceps sinensis fungus powder are very easy to be confused for sale and use due to similar appearances, and a rapid and effective method for identifying and distinguishing the two fungus powder does not exist at present. The method comprises the following steps: 1) preparing a standard control solution and a sample solution; 2) carrying out thin-layer sample application; 3) preparing a developing solvent; 4) unfolding; and 5) developing and identifying. The thin-layer chromatography identification method disclosed by the invention has the advantages of rapidness, high efficiency, simplicity in operation, low cost, visual and reliable detection result and the like, the fermented cordyceps sinensis bacterial powder and the fermented cordyceps sinensis bacterial powder can be rapidly and efficiently identified and distinguished, and the quality of the two bacterial powder is effectively ensured; and meanwhile, the blank that no rapid and effective method for identifying and distinguishing the fermented cordyceps sinensis fungus powder exists at present is filled up.

Description

technical field [0001] The invention relates to a thin-layer chromatography analysis method, in particular to a thin-layer chromatography analysis method for identifying and distinguishing fermented Cordyceps sinensis powder and fermented Cordyceps powder. Background technique [0002] Fermented Cordyceps sinensis powder (Cs-C-Q80) is the dry powder of the mycelium obtained by submerged liquid fermentation of Hirsntellasinensis L, which can be used for thinning under the Bailing Capsule item in the Chinese Pharmacopoeia. Layer chromatography was used for identification analysis. Fermented Cordyceps fungus powder (Cs-4) is obtained from Cordyceps sinensis (Cordycepssinensis (Berk) Sacc) isolated from Cordyceps - bat moth Paecilomyces ((Paecilomyceshepialichen) Cs-4) through submerged fermentation culture, the fermented product is filtered, and then The powder prepared by drying can be used for TLC identification and analysis under the item of Jinshuibao Tablets in the Chines...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94Y02A50/30
Inventor 高娟徐沛沛左志超
Owner SHAANXI JIAHE PHYTOCHEM
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