LC-MS (liquid chromatography-mass spectrometry)-based detection method for ten kinds of cardiovascular drug serum and kit thereof

Abstract

The invention discloses a detection kit for ten kinds of cardiovascular medicine serum based on LC-MS. The detection kit comprises a mixed standard stock solution, the solutes of the 10 kinds of mixed standard stock solutions are clopidogrel, rivaroxaban, apixaban, dabigatran, ticagrelor, rosuvastatin, dehydroxyethoxy ticagrelor, atorvastatin, o-hydroxy atorvastatin and p-hydroxy atorvastatin respectively. Chromatographic interference peaks of all the compounds are few, baseline separation can be achieved, specificity is high, reproducibility is good, and selectivity is good; the pretreatment method is simple and easy to operate, saves time and is far superior to the prior art.

Description

technical field [0001] The invention belongs to, specifically relates to a LC-MS-based detection method for 10 cardiovascular drug serums and a kit thereof. Background technique [0002] The blood concentration of cardiovascular drugs is easily affected by various factors such as heredity, food, and concomitant medication, and is closely related to the efficacy and toxicity. Too high blood concentration will cause liver and kidney toxicity and nervous system damage and infection. Rejection and induction of autoimmune diseases, and its renal toxicity is indistinguishable from rejection after renal transplantation. Therefore, monitoring the blood concentration of cardiovascular drugs is of great significance for guiding clinical rational drug use. [0003] At present, the commonly used methods for the determination of blood concentration of cardiovascular and other drugs are mainly enzyme immunoassay, high performance liquid chromatography and LC-MS / MS method. The enzyme immu...

AI Technical Summary

Problems solved by technology

[0005] First, the cost is high. At present, most HPLC-MS / MS methods use internal standards such as deuterium to correct matrix effects and other effects. The high cost of such internal standards will directly lead to high costs for the entire detection process;

[0006] Second, the throughput is low. The existing methods mostly measure one or several kinds of drugs at the same time, but rarely involve more than a dozen kinds, which affects the efficiency of detection;

[0007] Third, the sensitivity is low. In the process of detecting multiple drugs at the same time, there is a problem of relatively low sensitivity of multiple targets, which affects the accuracy of detection.

[0008] Fourth, the analysis time is long. The analysis time of the LC part accounts for the vast majority of the measurement methods in the prior art, resulting in a long time for drug detection. Generally, the analysis time is longer than 5 minutes.

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