Lactobacillus plantarum HOM3201 strain as well as viable bacteria preparation, preparation method and application of lactobacillus plantarum HOM3201 strain

A technology of HOM3201, Lactobacillus plantarum, applied in the field of microorganisms, can solve problems such as imperfection, and achieve the effects of increasing tolerance, good stability, and simple production process parameters

Pending Publication Date: 2022-07-15
科郦有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the regulation of blood sugar level by probiotics is not perfect and systematic

Method used

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  • Lactobacillus plantarum HOM3201 strain as well as viable bacteria preparation, preparation method and application of lactobacillus plantarum HOM3201 strain
  • Lactobacillus plantarum HOM3201 strain as well as viable bacteria preparation, preparation method and application of lactobacillus plantarum HOM3201 strain
  • Lactobacillus plantarum HOM3201 strain as well as viable bacteria preparation, preparation method and application of lactobacillus plantarum HOM3201 strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation and identification of Lactobacillus plantarum HOM3201 strain

[0038] (1) Lactobacillus plantarum strain screening medium formula

[0039] Modified MRS solid medium:

[0040] MRS medium (OXOID, CM1163) was added with 0.05 g of bromocresol green (Shanghai Shenggong) and 1 L of double distilled water, and stirred well. Adjust pH to 5.5, sterilize at 121 °C for 20 min, and set aside.

[0041] (2) Isolation and screening of Lactobacillus plantarum strains

[0042] a. Isolation and screening of HOM Lactobacillus plantarum strains

[0043] Weigh 1 g of kimchi, and make kimchi juice with 9 mL of 0.9% physiological saline. Aspirate 1 mL of pickle juice, and dilute the sample by 10-fold dilution method, with a dilution of 10 -3 -10 -5 . The diluted kimchi juice was spread on the above-mentioned modified MRS plate and cultured anaerobically at 35°C for 72h. Pick out a single colony with a moist and smooth surface, neat edges, milky yellow or milky whit...

Embodiment 2

[0058] Example 2 Gastrointestinal tract passing ability test

[0059] (1) Isolation and activation of strains

[0060] In this experiment, three commercial strains of Lactobacillus plantarum 299V (isolated from Jarrow probiotic products, and the isolation method is the same as that of b. The isolated strains Lactobacillus plantarum Lp-115 and Lactobacillus plantarum ST-III were used as positive controls. Each strain was inoculated into MRS liquid medium, cultured at 37° C. for 24 h, activated twice, and used for later use.

[0061] (2) Preparation of artificial gastric juice

[0062] Take 16.4 mL of dilute hydrochloric acid and 10 g of pepsin, add about 800 mL of water, shake well, adjust the pH to 3.0, add water to make up to 1 L, and filter with a 0.22 μm microporous membrane for later use.

[0063] (3) Preparation of artificial intestinal juice

[0064] Take 6.8 g of potassium dihydrogen phosphate, add 500 mL of water to dissolve, adjust the pH to 6.8 with 0.1 mol / L sod...

Embodiment 3

[0076] Embodiment 3 Inhibition of common pathogenic bacteria ability test

[0077] (1) Activation of pathogenic bacteria

[0078] Five pathogenic bacteria were selected in this test: Escherichia coli (E.coli) ATCC8739, Salmonella typhimurium ATCC14028, Staphylococcus aureus ATCC6538, Pseudomonas aeruginosa ATCC9027, single Listeria monocytogenes ATCC19111. These pathogenic strains were purchased from ATCC (American type culture collection).

[0079] The pathogenic strains were respectively inoculated into nutrient agar medium, and cultured with shaking at 200rpm at 37°C for 12h. The indicator bacteria were adjusted to OD=0.1 with fresh medium for use.

[0080] (2) Activation of lactic acid bacteria strains

[0081] In this experiment, commercial strains Lactobacillus plantarum 299V (separation method is the same as Example 1 and 2), Lp-115 (separation method is the same as Example 1), ST-III strain (separation method is the same as Example 1) and Lactobacillus rhamnosus (La...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum HOM3201 strain, a viable bacterium preparation containing the lactobacillus plantarum HOM3201 strain as well as a preparation method and application of the lactobacillus plantarum HOM3201 strain. The preservation number of the lactobacillus plantarum HOM3201 strain is CGMCC (China General Microbiological Culture Collection Center) No. 22700. The lactobacillus plantarum HOM3201 strain can significantly reduce the fasting blood glucose level of insulin-resistant glucose/lipid metabolism disorder model rats, increase the tolerance of the body to glucose, and reduce the insulin resistance index.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactobacillus plantarum HOM3201 strain, a live bacteria preparation comprising the same, and a preparation method and application thereof. Background technique [0002] The World Health Organization (WHO) defines probiotics as live microorganisms that, when consumed in sufficient amounts, are beneficial to the host. Only strains that have been scientifically proven to be beneficial to health can be called "probiotics". The core characteristics of probiotics are sufficient quantity, viable state and beneficial health functions. Lactobacillus plantarum is a kind of ubiquitous probiotics, which are often isolated from fermented foods such as sauerkraut and kimchi, as well as in the human body. Lactobacillus plantarum has beneficial functions in improving intestinal barrier, diabetes, hypertension, obesity, anti-infection, mental illness, etc. [0003] The incidence of ty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P3/10C12R1/25
CPCC12N1/20A23L33/135A61K35/747A61P3/10A23V2002/00A23V2400/169A23V2200/328
Inventor 张迪张世奇葛阳阳张晶晶王婷婷李守远林钟润
Owner 科郦有限公司
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