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Fluorescence compensation method and system for quantitative analysis of cells

A quantitative analysis and compensation method technology, applied in the field of fluorescence compensation method and its system, can solve the problems that the accuracy of the fluorescence signal intensity value cannot be guaranteed, and achieve the effect of small error and simple calculation

Pending Publication Date: 2022-07-15
青岛瑞斯凯尔生物科技有限公司
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Problems solved by technology

[0005] The embodiment of the present invention provides a fluorescence compensation method and system for quantitative analysis of cells, the purpose of which is to solve the problem that the accuracy of the real fluorescence signal intensity values ​​of various fluoresceins cannot be guaranteed in the existing automatic compensation of flow cytometers The problem

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  • Fluorescence compensation method and system for quantitative analysis of cells
  • Fluorescence compensation method and system for quantitative analysis of cells
  • Fluorescence compensation method and system for quantitative analysis of cells

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Embodiment 1

[0057] Refer to the attached figure 1 As shown, the present invention provides a technical solution: a fluorescence compensation method for quantitative analysis of cells, comprising the following steps:

[0058]In step S1, the fluorescence signal intensity data of each measurement channel is obtained and the signal intensity waveform is drawn.

[0059] Wherein, it specifically includes: collecting the fluorescence signal intensity data of each of the measurement channels from the lower computer in a specific time period;

[0060] The signal intensity waveform is drawn according to the fluorescence signal intensity data of each measurement channel.

[0061] Specifically, before the acquisition, the lower computer first collects and stores the fluorescence signal intensity data; wherein, the lower computer is specifically composed of PMT photoelectric sensor, A / D converter, memory, main control chip and communication module, PMT photoelectric sensor, A The / D converter, the m...

Embodiment 2

[0103] The embodiment of the present invention also discloses a fluorescence compensation system for quantitative analysis of cells. figure 2 shown, including:

[0104] an obtaining module 100 for obtaining fluorescence signal intensity data of each measurement channel and drawing a signal intensity waveform;

[0105] A filtering module 200, configured to perform filtering processing on the signal strength waveform to extract an effective peak value;

[0106] a calculation module 300 for calculating the signal value of each of the measurement channels according to the effective peak value;

[0107] The first processing module 400 is used to calculate the crosstalk coefficients of various fluorescein in each of the measurement channels one by one, and obtain a fluorescence compensation matrix after calculation; wherein, it specifically includes:

[0108] an acquisition unit, used to obtain the signal values ​​of each of the fluorescein in all the measurement channels;

[01...

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Abstract

The invention provides a fluorescence compensation method and system for quantitative analysis of cells, and belongs to the technical field of flow data analysis, and the fluorescence compensation method for quantitative analysis of cells comprises the following steps: obtaining fluorescence signal intensity data of each measurement channel and drawing a signal intensity waveform; filtering the signal intensity waveform to extract an effective peak value; calculating a signal value of the measurement channel according to the effective peak value; calculating crosstalk coefficients of various fluoresceins in the measurement channels one by one, and obtaining a fluorescence compensation matrix after calculation; by measuring and calculating the proportion of each fluorescein in all measurement channels, a fluorescence compensation matrix can be obtained, so that the real fluorescence signal intensity values of various fluoresceins can be simply and conveniently calculated, and the error of the real fluorescence signal intensity values of various fluoresceins can be smaller and closer to an accurate value.

Description

technical field [0001] The invention belongs to the technical field of flow data analysis, and in particular, relates to a fluorescence compensation method and a system for quantitative analysis of cells. Background technique [0002] Flow cytometer is a new high-tech instrument integrating laser technology, electronic physics technology, photoelectric measurement technology, electronic computer technology, cell fluorescence chemical technology and monoclonal antibody technology. For single cells or other particles in high-speed, linear flow in suspension, by detecting scattered light signals and / or labeled fluorescent signals, high-speed one-by-one multi-parameter quantitative analysis is achieved. It has a wide range of applications in the fields of cell biology, cell cycle dynamics, immunology, hematology and oncology. [0003] When performing flow cytometry analysis, the samples to be tested usually carry two or more fluoresceins, such as phycoerythrin (PE), fluorescein...

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1436G01N2015/144G01N15/1433
Inventor 强茂纪存朋林文革谢万龙孙谧
Owner 青岛瑞斯凯尔生物科技有限公司
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