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Metabolic marker combination for mutton producing area traceability, screening method and application

A technology of metabolic markers and screening methods, which is applied in the field of food authenticity detection and identification, can solve the problems of complex pretreatment and large sampling volume, and achieve the effect of simple detection method, strong universal applicability, and suitable for popularization and application

Pending Publication Date: 2022-07-22
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these traditional detection techniques for origin traceability of mutton have disadvantages such as complicated pre-processing or large sampling volume, and it is urgent to develop a rapid and reliable method for rapid traceability of mutton origin with less sampling amount, so as to maintain market fairness and protect consumer rights.

Method used

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  • Metabolic marker combination for mutton producing area traceability, screening method and application
  • Metabolic marker combination for mutton producing area traceability, screening method and application
  • Metabolic marker combination for mutton producing area traceability, screening method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] This example provides a screening method for a combination of metabolic markers, using a real-time direct analysis ion source coupled with a quadrupole-time-of-flight mass spectrometer (DART-TOFMS) to perform metabolomics data on the metabolites of mutton samples from four origins Collecting, performing chemometric analysis on the detection data, extracting characteristic information, and establishing an analysis model to determine the differential metabolites of mutton from four origins include the following steps:

[0064] 1. Source of mutton samples

[0065] In this example, Ningxia Tan mutton samples, Gansu black goat meat samples, New Zealand lamb samples and Anhui Lake mutton samples were selected for screening of metabolic markers, and 30 samples from each of the four origins were collected, with a total of 120 lamb samples aged 6-8 months. See Table 1 for details. Mixed breed lambs are raised with their ewes until they reach weaning weight. As the sampling sit...

Embodiment 2

[0083] In this example, the combination of metabolic markers provided in Example 1 is used to perform traceability analysis on the four groups of samples in Example 1, and the combination of 26 compounds obtained in Example 1 is used to reduce the cost of the four sources of mutton selected in Example 1. Do a variance analysis.

[0084] For different sample groups, the LDA models constructed with 26 compounds are as follows:

[0085] Anhui sample group:

[0086] Y 1 =-31.192+X 1 ×0.003+X 2 ×0.00002454+X 3 ×0+X 4 ×0.001+X 5 ×0+X 6 ×0-X 7 ×0.002+X 8 ×0.001+X 9 ×0.00003464+X 10 ×0.002-X 11 ×0.00001767+X 12 ×0.00002281+X 13 ×0.000009942+X 14 ×0.002+X 15 ×0+X 16 ×0.014-X 17 ×0.001+X 18 ×0.003+X 19 ×0+X 20 ×0+X 21 ×0.00004071+X 22 ×0.001-X 23 ×0.00003265+X 24 ×0+X 25 ×0.001+X 26 ×0.002.

[0087] New Zealand sample set:

[0088] Y 2 =-43.451+X 1 ×0.002+X 2 ×0.000001113+X 3 ×0+X 4 ×0.001+X 5 ×0+X 6 ×0-X 7 ×0.001+X 8 ×0.001+X 9 ×0.00006291+X 10 ×...

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Abstract

The invention relates to the technical field of food authenticity detection and identification, in particular to a metabolic marker combination for mutton origin traceability, a screening method and application. The metabolic marker combination for mutton origin traceability provided by the invention is obtained by combining metabonomics data and a chemometrics method, provides a scientific basis for mutton origin traceability research, authenticity identification and quality safety control, has strong universality, and is suitable for popularization and application. The mutton origin traceability detection method provided by the invention is simple, rapid, accurate and stable, and the non-targeted metabonomics technology based on the real-time direct analysis ion source and quadrupole-flight time high-resolution mass spectrum coupling technology is applied to the field of mutton origin traceability for the first time.

Description

technical field [0001] The invention relates to the technical field of food authenticity detection and identification, in particular, to a combination of metabolic markers, a screening method and an application for tracing the origin of mutton. Background technique [0002] With the improvement of economic living standards, consumers have higher and higher requirements for the quality of agricultural products. Lamb is becoming increasingly popular with consumers as an excellent source of animal protein. Due to the excellent quality and unique flavor of GI lamb and high-quality domestic and foreign mutton, consumers are more willing to buy. Mutton is included in the first batch of 100 GI products in the China-EU Mutual Recognition GI Catalog. Most consumers who buy mutton are most concerned about the authenticity of agricultural products, because it not only involves the price of mutton, but also the quality and safety of mutton. Authenticity labels can influence consumers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 赵燕郄梦洁赵姗姗马玉轩胡翔宇张娟
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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