Electrospray ionization mass spectrometry device and electrospray method
An electrospray mass spectrometry and electrospray technology, which is applied in the direction of measuring devices, circuits, discharge tubes, etc., can solve problems such as capillary outlet blockage, signal suppression of analytes, detection sensitivity and detection accuracy reduction, etc.
Pending Publication Date: 2022-07-26
INST OF CHEM CHINESE ACAD OF SCI
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Problems solved by technology
[0005] The inventors found that in the process of mass spectrometry detection, actual biological samples such as human body fluids, cells, etc. generally contain a relatively high concentration of salt, and the electrospray method in the related art usually faces the problem of salt crystallization at the liquid end of the capillary when detecting samples. Problems such as clogging and the signal of the analyte being suppressed by salt clusters make the detection sensitivity and detection accuracy significantly reduced
Method used
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Embodiment 1
[0051] The solution to be tested is 0.01mol / L phosphate buffer solution, the zero peak value of the first power supply treatment is 3200V, the frequency of the first power supply treatment is 20kHz, the time of the first power supply treatment is 50μs, and the pulse width of the first power supply treatment is 10 μs; the zero peak value of the second power supply processing is 800V, the frequency of the second power supply processing is 20 kHz, the second power supply processing time is 50 μs, and the pulse width of the second power supply processing is 5 μs. During the detection process, the first power supply processing and the second power supply processing are alternately performed in sequence.
Embodiment 2
[0053] Example 2 is the same as Example 1, the difference is that the solution to be tested is an arginine solution containing 5 mmol / L phosphate buffer solution, wherein the concentration of arginine is 0.8 mg / L.
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Abstract
The invention discloses an electrospray ionization mass spectrometry device and an electrospray method, the electrospray ionization mass spectrometry device comprises an electrospray nozzle and a pulse power supply, the electrospray nozzle comprises a capillary tube and a first electrode, the capillary tube comprises a liquid injection end and a liquid outlet end, the first electrode is arranged in the capillary tube, and the pulse power supply is arranged in the first electrode. The capillary tube extends out of the liquid injection end of the capillary tube and is electrically connected with the pulse power supply, and the pulse power supply is a positive and negative pulse power supply. Therefore, rapid online desalination of the electrospray ion source can be realized.
Description
technical field [0001] The present invention relates to the technical field of mass spectrometry related applications, in particular, to an electrospray mass spectrometry device and an electrospray method. Background technique [0002] Nanoelectrospray is an ionization technique used in mass spectrometers. Compared to conventional electrospray ion sources, nano-electrospray has a lower flow rate, thus enabling higher sample economy and detection sensitivity. In the actual detection process, biological samples need to undergo pretreatment to improve detection sensitivity and accuracy, but too many pretreatment steps will increase sample consumption and reduce detection speed, resulting in excessive sample consumption and long detection time. question. [0003] Therefore, the current electrospray mass spectrometry devices and electrospray methods still need to be improved. SUMMARY OF THE INVENTION [0004] The present invention is made based on the inventors' findings and...
Claims
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IPC IPC(8): H01J49/04H01J49/16H01J49/00G01N27/62
CPCH01J49/0445H01J49/165H01J49/167H01J49/0027H01J49/0031G01N27/62
Inventor 聂宗秀李玉泽王逸冉熊彩侨刘会会
Owner INST OF CHEM CHINESE ACAD OF SCI