Application of 2-methoxynaphthalene in preparation of product for resisting skin light injury

A technology of methoxynaphthalene and photodamage, which is applied in the field of biomedicine to achieve the effect of improving skin wrinkle formation and cell oxidative damage

Pending Publication Date: 2022-07-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of 2-methoxynaphthalene in improving UVB-induced cell oxidative damage and anti-skin photoaging has not been reported yet.

Method used

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  • Application of 2-methoxynaphthalene in preparation of product for resisting skin light injury
  • Application of 2-methoxynaphthalene in preparation of product for resisting skin light injury
  • Application of 2-methoxynaphthalene in preparation of product for resisting skin light injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Induction of photoaging damage in dermal fibroblasts Hs 68

[0035] Hs 68 cells were seeded in 96-well plates, and when grown to a density of 50%, 0, 3, 5, 7, 10, 20, 30 mJ / cm were administered 2 After culturing for 24 hours, the cell viability under different UV irradiation doses was detected with Biyuntian CCK8 kit.

[0036] Experimental results ( figure 1 ) showed that the cell viability decreased gradually with the increase of UVB irradiation dose. The UVB irradiation dose should be more than 90% of the cell survival rate, so the UVB dose is selected as 5mJ / cm 2 used for subsequent experiments.

Embodiment 2

[0038] Toxicity of 2-methoxynaphthalene on Hs 68 cells

[0039] Hs 68 cells were seeded in 96-well plates, and when the density reached 50%, they were treated with 0, 12.5, 25, 50, and 100 μM 2-methoxynaphthalene (with DMSO as the solvent), and were incubated for 24 hours. Biyuntian CCK8 kit to detect cell viability at different doses.

[0040] according to figure 2 As a result, 2-methoxynaphthalene was not toxic to Hs 68 cells within the experimental concentration range, so the maximum non-toxic concentration within the experimental range of 100 μM was selected for subsequent experiments.

Embodiment 3

[0042] Effects of 2-Methoxynaphthalene on MMP-1 Protein Expression

[0043] The cells were seeded in a 6-well plate, and when the density reached 50%, they were treated with UVB, UVB+ 2-methoxynaphthalene at a concentration of 100 μM, and UVB+0.1% DMSO, while the cells in the blank control group did not receive any treatment (marked as UVB0); after each group continued to culture for 24 hours, the supernatant and cells were collected.

[0044] MMP-1 protein was detected by elisa kit. ELISA detection principle: The MMP-1 detection antibody was immobilized on the surface of polystyrene microplate by physical adsorption, and the sample to be tested was added, which was indirectly reflected by the color of the enzyme marker. MMP-1 content. Finally, MMP-1 protein levels were normalized to total cellular protein content.

[0045] The results show( image 3), after Hs 68 cells were irradiated with UVB, the level of MMP-1 protein secreted by Hs 68 cells increased significantly ( *...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly discloses application of 2-methoxynaphthalene in preparation of a product for resisting skin light injury. Experimental results prove that the 2-methoxynaphthalene can improve the UVB-induced cell oxidative damage by down-regulating the secretion level of MMP-1 protein and the expression level of MMP-1 genes and MMP-9 genes in UVB-induced oxidative damage cells and up-regulating the secretion level of I-type collagen and the expression level of COL1A1 genes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically discloses the use of 2-methoxynaphthalene in preparing an anti-skin photodamage product. Background technique [0002] Skin is the largest organ of the human body, and skin aging is one of the main manifestations of body aging. Skin photoaging caused by ultraviolet rays is the most critical factor causing skin aging damage. Photoaging skin is characterized by roughness, thickening and dryness of the exposed parts of the skin, sagging skin, deepening and thickening of wrinkles, local hyperpigmentation or telangiectasia, and may even appear various benign or malignant tumors (such as solar keratosis, squamous cell carcinoma, malignant melanoma, etc.). [0003] The skin is the body's first defensive barrier, covering the entire body. As the largest organ, the skin makes up about 16% of the human body. The skin area of ​​an adult is about 1.2-2.0m 2 . The skin is in direct ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/085A61K8/33A23L33/10A61P17/16A61Q19/00A61Q19/08
CPCA61K31/085A61P17/16A23L33/10A61K8/33A61Q19/08A61Q19/004A23V2002/00A23V2200/318
Inventor 仝涛黄昆仑耿睿璇
Owner CHINA AGRI UNIV
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