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Scutellaria baicalensis leaf extract, preparation method and application thereof

A technology of scutellaria baicalensis leaves and extracts, which is applied in the field of extraction of non-medicinal parts of Chinese herbal medicines, can solve the problems that the comprehensive development and utilization of scutellaria baicalensis leaves have not fully improved the economic value of scutellaria baicalensis leaves, and improve learning and memory ability and oxidative damage , the effect of increasing economic value

Inactive Publication Date: 2020-01-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are few research reports on the specific functional substances in Scutellaria baicalensis leaves, so the comprehensive development and utilization of Scutellaria baicalensis leaves and the economic value of Scutellaria baicalensis leaves have not reached a comprehensive improvement

Method used

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  • Scutellaria baicalensis leaf extract, preparation method and application thereof
  • Scutellaria baicalensis leaf extract, preparation method and application thereof
  • Scutellaria baicalensis leaf extract, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: The preparation method of Scutellaria baicalensis leaf extract: Scutellaria baicalensis leaf 400g, water temperature 89 ℃, add 15 times the quality of water for the first time and decoct for 1 hour, filter; add 10 times the quality of water for the second time and decoct for 1 hour, filter, The combined filtrates were concentrated, and then the concentrated solution was freeze-dried to obtain the crude extract of Scutellaria baicalensis leaves. The crude extract of Scutellaria baicalensis obtained above was separated by HPD100 macroporous adsorption resin, and water, 50% ethanol and 95% ethanol were used as elution solvents respectively, and eluted at a flow rate of 3 BV / h, and 3 BV were collected for each gradient. The 50% ethanol and 95% ethanol eluate were concentrated under reduced pressure and freeze-dried to obtain the extract of Scutellaria baicalensis leaves.

Embodiment 2

[0020] Example 2: Analysis of chemical components of Scutellaria baicalensis leaf extract: The main components of Scutellaria baicalensis leaf extract were identified by UHPLC-Q Exactive Orbitrap-MS analysis instrument and HSS T3 chromatographic column analysis. Through the comparison of reference substances and databases, combined with existing literature reports, a total of 10 main chemical components were identified. figure 1 .

[0021] Liquid phase conditions: mobile phase: A (acetonitrile), B (water, containing 0.1% formic acid); mobile phase gradient: 0 ~ 4 min, 15% ~20%A; 4 ~ 8 min, 20~23% A; 8 ~10min, 23~25%A; 10~14min, 25~50%A; 14~18min, 50~52%A; 18~20min, 52~90%A; 20~22min, 90%A ; 22 ~ 22.5min, 90 ~ 15% A; 22.5 ~ 25min, 15% A. Flow rate 0.30 ml / min, 2 μL injection volume, 40 °C column temperature, Waters ACQUITY UPLC HSS T3 column (2.1 × 100 m, 1.8 m).

[0022] Mass spectrometry conditions: Ionization mode using ESI electrospray negative ion detection, scan mode:...

Embodiment 3

[0024] Example 3: Study on the effect of Scutellaria baicalensis leaf extract on aging rats

[0025] 1. 40 SPF-grade male SD rats were purchased from Beijing Weitong Lihua Co., Ltd., with a body weight of (180±20) g. The humidity in the animal laboratory is (50±10)%, the room temperature is (25±1)°C, and the light cycle is every 12 hours. Five mice are placed in each cage, and they can drink water and eat freely during the experiment.

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Abstract

The invention belongs to the technical field of extraction of non-medicinal parts of traditional Chinese medicinal materials, and provides a scutellaria baicalensis leaf extract, a preparation methodand application thereof in order to improve the utilization rate of scutellaria baicalensis leaves. The scutellaria baicalensis leaf extract is applied to anti-aging. The scutellaria baicalensis leafextract is repeatedly decocted and extracted from the scutellaria baicalensis leaves with water, extracting solutions are combined, concentration under reduced pressure and freeze-drying are conducted, then the extracting solutions are separated by HPD100 macroporous adsorption resin, and water, 50% of ethyl alcohol, and 95% of ethyl alcohol are used as elution solvents. The eluate of 50% of the ethyl alcohol and 95% of the ethyl alcohol is obtained by concentration under reduced pressure. Pharmacodynamic experiments proves that the scutellaria baicalensis leaf extract can improve the learningand memory ability and oxidative damage in aging rats, and the scutellaria baicalensis leaf extract can be used for developing health foods and medicines with anti-aging as the main function.

Description

technical field [0001] The invention belongs to the technical field of extracting non-medicinal parts of traditional Chinese medicinal materials, and in particular relates to a scutellaria baicalensis leaf extract and a preparation method thereof, and the application of the scutellaria baicalensis leaf extract in anti-aging. Background technique [0002] Lamiaceae Scutellaria baicalensis ( Scutellaria baicalensis Georgi) is often used as medicine from its root, which has the effects of clearing away heat and dampness, purging fire and detoxification, and stopping bleeding. Its main active ingredients, flavonoids, have pharmacological effects such as antibacterial, antiviral, antipyretic, analgesic, anti-inflammatory, anti-tumor, and antioxidant . The leaves, as the aerial part of Scutellaria baicalensis, grow luxuriantly and have a large yield. [0003] Efficacy and role of Scutellaria baicalensis: Antibacterial and antiviral is one of the important functions of scutellar...

Claims

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Application Information

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IPC IPC(8): A61K36/539A61P39/06
CPCA61K36/539A61K2236/331A61K2236/39A61K2236/51A61K2236/55A61P39/06
Inventor 周玉枝宋佳秦雪梅张丽增杜冠华
Owner SHANXI UNIV
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