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Application of eupatilin in preparation of product for resisting skin light injury

A technology of eupholzanthin and photodamage, which is applied in the field of biomedicine to improve skin wrinkle formation and cell oxidative damage

Pending Publication Date: 2022-05-06
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of eupholzanthin in improving UVB-induced cell oxidative damage and anti-skin photoaging has not been reported yet.

Method used

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  • Application of eupatilin in preparation of product for resisting skin light injury
  • Application of eupatilin in preparation of product for resisting skin light injury
  • Application of eupatilin in preparation of product for resisting skin light injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Induction of photoaging damage in HaCaT cells

[0037] HaCaT cells were seeded in 96-well plates, and when they grew to a density of 50%, 0, 3, 5, 7, 10, 20, 30 mJ / cm 2 After continuing to culture for 24 hours, use Beyontian CCK8 kit to detect the cell survival rate under different ultraviolet radiation doses.

[0038] figure 1 It was shown that with the increase of UVB radiation dose, the survival rate of cells decreased gradually. The dose of UVB radiation should be above 80% of the cell survival rate, so choose the dose of UVB as 5mJ / cm 2 for subsequent experiments.

Embodiment 2

[0040] Toxicity of eupholzanthin to HaCaT cells

[0041] HaCaT cells were seeded in a 96-well plate, and when they grew to a density of 50%, they were treated with 0, 12.5, 25, 50, and 100 μM concentrations of eupholzanthin (using DMSO as a solvent), and after continuing to culture for 24 hours, they were treated with Phi Yuntian CCK8 kit is used to detect the cell survival rate under different doses.

[0042] according to figure 2 As a result, based on the cell viability of 90% as the standard, the maximum non-toxic concentration within the experimental range was 25 μM. At the same time, combined with the literature reports on the toxicity of eupholzanthin, subsequent experiments were carried out at a concentration of eupholzanthin of 30 μM.

Embodiment 3

[0044] Improvement effect on UVB-induced oxidative damage of HaCaT cells

[0045] Reactive oxygen species (ROS), lipid peroxidation product malondialdehyde (MDA), antioxidant enzyme superoxide dismutase (SOD) were used as indicators for research.

[0046] 1. Reactive oxygen species (ROS)

[0047]HaCaT cells were seeded in a 96-well plate, and when they grew to a density of 50%, they were treated with UVB, UVB+ with a concentration of 100 μM eupholzanthin, and UVB+0.1% DMSO, while the cells in the blank control group were not treated with any treatment (marked as UVB0); after each group continued to culture for 24 hours, the Beyontian ROS detection kit was used to detect the improvement effect of eupholzanthin on UVB-induced ROS accumulation.

[0048] The intracellular ROS level of HaCaT cells was characterized by the fluorescence intensity of DCFH-DA probe. image 3 It was shown that after HaCaT cells were irradiated with UVB, the level of intracellular ROS was significantly...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly discloses application of eupatilin in preparation of a product for resisting skin light injury. Experimental results prove that eupatilin can improve the UVB-induced cell oxidative damage by reducing the expression levels of ROS, MDA and SOD in UVB-induced oxidative damage cells and reducing the relative expression quantity of MMP-1, MMP-3 and MMP-9 and the expression quantity of MMP-1 protein.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically discloses the use of eupholzanthin in preparing anti-skin photodamage products. Background technique [0002] The skin is the largest organ of the human body, and skin aging is one of the main manifestations of body aging. Skin photoaging caused by ultraviolet rays is the most critical factor causing skin aging damage. Photoaging skin is characterized by roughness, thickening, and dryness of the skin exposed parts, sagging skin, deepening and thickening of wrinkles, local hyperpigmentation or telangiectasia, and even various benign or malignant tumors (such as solar keratosis, squamous cell carcinoma, malignant melanoma, etc.). [0003] The skin is the first defense barrier of the human body and covers the whole body. As the largest organ, the skin accounts for about 16% of the human body. The skin area of ​​an adult is about 1.2-2.0m 2 . The skin is in direct contact w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A23L33/10A61P17/18A61P17/16
CPCA61K31/352A61P17/18A61P17/16A23L33/10A23V2002/00A23V2200/318
Inventor 仝涛黄昆仑耿睿璇
Owner CHINA AGRI UNIV
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