Method for detecting content of animal-derived natural pigment in food
A technology of natural pigments and detection methods, applied in the food field, can solve the problems of fluorescence quenching, interference, poor sensitivity and recovery rate, etc., and achieve the effect of simple and fast method, good peak shape and sensitivity, and reduced solvent consumption.
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[0027] This embodiment case is implemented on the premise of the above-mentioned technology of the present invention, and now provides detailed implementation mode and specific operation process to illustrate the present invention:
[0028] 1 Reagents and materials
[0029] Unless otherwise specified, all reagents are of analytical grade, and the water is first-grade water specified in GB / T 6682.
[0030] 1.1 Methanol: chromatographically pure.
[0031] 1.2 Ammonia
[0032] 1.3 Ammonium acetate
[0033] 1.4 Acetic acid
[0034] 1.5 PWAX SPE Cartridge: 60 mg / 3mL
[0035] 1.6 Polyamide solid phase extraction column: self-packing column, 500 mg / 6 mL
[0036] 1.7 Standard: Lac red pigment standard (CAS No.60687-93-6, purity 100%).
[0037] 1.8 Preparation of standard stock solution: Accurately weigh 10 mg of standard lac red pigment, dissolve it in methanol and dilute to 10 mL, prepare a standard stock solution with a concentration of 1000 mg / L, and store it in a refrigerato...
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