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Method for measuring content of yam saponin

A technology of diosgenin and a determination method, which is applied in the field of gas chromatography determination, can solve the problems of long period, inapplicability, uneconomical large-scale production, etc., and achieves the effects of simple operation and low cost

Inactive Publication Date: 2005-02-02
郭长合
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, there is no assay method for diosgenin content in the national standard, and the gravimetric method is mainly used in production. This method cannot accurately judge the content of diosgenin, and can only be roughly estimated; there are also thin-layer analysis methods for determination. The method can only be roughly compared with the standard sample, and the value is not accurate, so it is not applicable in actual production; if liquid chromatography is used to measure, the value is accurate, but the cycle is long, the analysis cost is high, and large-scale production is uneconomical

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  • Method for measuring content of yam saponin

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Experimental program
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Embodiment Construction

[0012] 1. Reagents and solutions

[0013] Diosgenin standard sample: known content;

[0014] Internal standard: didecyl phthalate, should not contain impurities that interfere with analysis;

[0015] Internal standard solution: Weigh 1.0 g of didecyl phthalate (accurate to 0.0002 g) in a 100 mL container bottle; dilute to the mark with absolute ethanol and shake well;

[0016] Absolute ethanol: analytically pure;

[0017] Fixative: SE-30;

[0018] Carrier: Chromosorb W-HP (150 μm ~ 180 μm).

[0019] 2. Instrument

[0020] Gas chromatograph: with hydrogen flame ionization detector and chromatographic data processor;

[0021] Injector: 10 μL;

[0022] Chromatographic column: 1m×3mm(id) glass column or stainless steel column;

[0023] Column packing: SE-30 coated in Chromosorb W-HP.

[0024] The packing of the column is carried out as usual.

[0025] 3. Determination steps

[0026] 1. Configuration of standard sample solution Weigh 0.04g of diosgenin standard product, a...

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Abstract

A method for measuring dioscin content uses didecyl phthalate as internal reference, SE-30 as fixing liquid, Chromosorb W-HP as carrier and N2 as gas carrier, and includes dissolving the specimen in absolute alcohol, and gas-phase chromatographic separation and measuring with glass column or stainless steel column filled by SE-30 / Chromosorb W-HP. Its advantages are high correctness and speed, and low cost.

Description

(1) Technical field [0001] The invention belongs to a gas chromatography determination method, in particular to a determination method of diosgenin content. (2) Background technology [0002] At present, there is no assay method for diosgenin content in national standards, and gravimetric method is mainly used in production. This method cannot accurately determine the content of diosgenin, and can only be roughly estimated; there are also thin-layer analysis methods. The method can only be roughly estimated by comparison with the standard sample, and the value is not accurate, so it is not applicable in actual production; if liquid chromatography is used for measurement, the value is accurate, but the cycle is long, the analysis cost is high, and large-scale production is uneconomical. (3) Contents of the invention [0003] The technical problem mainly solved by the invention is: the content of diosgenin can be accurately and rapidly determined; the determination method is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/68
Inventor 张瑜郭长合
Owner 郭长合