Surface tension driving liquid flow chiplized high-density micro-array liquid transferring equipment

Abstract

The invention relates to a chip high density micro-array liquid transferring apparatus comprising a sheet shape carrier with micro-array liquid transferring combination, the combination includes liquid storing portion, liquid transferring head and micro-channel, the micro-channel is linked between the liquid storing portion and the liquid transferring head. By using chip high density micro-array liquid transferring structure, and since the micro-liquid flow of the microscale liquid transferring for the whole apparatus only depends on the acting of surface tension, the manufacturing cost is lower, and the reproducibility of the transferring volume is good.

Description

technical field [0001] The invention relates to a chip-based high-density microarrayer technique on chip device. The device integrates thousands of groups of liquid transfer combinations on a chip, and is called a microarrayer technique on chip. Transfer chip (microarrayer chip), which is a high-density array with precise volume control, high-efficiency and low-cost ultra-micro liquid transfer device. Background technique [0002] Biological / chemical microarray (Biological / chemical microarray) refers to the use of industrial technologies such as microelectronics and micromechanics to immobilize biological or chemical molecules in a certain position on solid-phase carriers such as glass, silicon wafers, and plastics, and to make them used in The product of biological / chemical analysis, its target can be gene, protein or cell tissue, etc. The currently developed biological / chemical microarray products include Genechip (DNAchip or Microarray), protein chip (protein chip), micr...

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is that the expensive needle point is easy to damage and the uniformity of sample points is relatively poor (Chinese patent CN1302904), although people have designed many kinds of sampling needles (U.S. Patent Augenlicht4,981,783; Drmanac et al., U.S. Patent 5,525,464; Roach, U.S. Patent 5,770,151; Brown et al., U.S. Patent 5,807,522; Chinese Patent CN1302904) to make up for these shortcomings, utilize this method to make high-density chips, but its mechanical structure makes the number of this method sampling pins very few (usually 32 Needle), the efficiency is limited, and it takes a long time to make a high-density biological / chemical microarray, especially when different samples are placed on a chip, it takes a lot of time to clean and dry the needle tip, so it cannot meet the increasingly high requirements. Density requirements for chip development trends (US Patent 6,245,297)

Images