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Method for producing fucan sulfatase by means of bacteria

A technology of fucoidan sulfate and bacteria, applied in biochemical equipment and methods, bacteria, enzymes, etc., can solve the problems of complex culture components, difficult industrial production, long enzyme production time, etc., and achieve high enzyme activity and low cost Low, good stability

Inactive Publication Date: 2005-06-15
OCEAN UNIV OF CHINA
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Problems solved by technology

However, it takes a long time to produce enzymes with these strains, the enzyme activity of the output is low, and the culture components are complicated, so it is difficult to be suitable for industrial production.

Method used

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Embodiment Construction

[0006] Vibrio riverus used in the present invention Vibrio fluvialis The strain is arc-shaped, terminal single flagella, G - , colony round, milky white. The medium used to cultivate the bacteria is seawater bacterial medium 2216E. The components of the medium are 1 gram of yeast extract, 5 grams of peptone, 0.01 gram of ferric phosphate, 15 grams of agar, 1000 ml of aged seawater, and its pH is 7.6-7.8. The enzyme-producing medium used in the present invention is the above-mentioned medium, and in addition, 0.8-1.5% (by weight, the same below) of seaweed powder is added, and the enzyme liquid can be obtained by culturing for 20 hours. The depository unit of the above-mentioned Vibrio river is China Center for Type Culture Collection, referred to as CCTCC, address: Wuhan, China, Wuhan University, and the date of deposit is June 5, 2000. The deposit number is M200015.

[0007] After activating Vibrio riverina with the above-mentioned 2216E medium, insert 5% of the inoculum i...

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Abstract

A method for producing fucoidan sulfatase by bacteria, comprising: inserting the bacterial strain into a fermentation medium containing seaweed powder, cultivating it at a certain temperature for a certain period of time, and then putting in an enzyme extracting agent to sink the produced sediment , and then separated, finally freeze-dried, characterized in that the strain used is Vibrio fluvialis. The bacterial species used in the present invention has the characteristics of wide range of nutritional requirements, easy cultivation and short generation time, especially high enzyme-producing activity, and the enzyme activity of the fermented liquid is 226U / ml. The fucoidan sulfatase produced by the method of the present invention is endocut and exocut, and the fucoidan sulfate can be degraded to obtain fucoidan sulfate oligosaccharides, disaccharides and monosaccharides. The enzyme product has high activity, good stability and low cost, and can realize industrial production.

Description

technical field [0001] The invention relates to a method for producing fucoidan sulfatase by bacteria. Background technique [0002] As far as the inventors know, fucoidan sulfatase shows an extremely important effect in the preparation of fucoidan sulfate oligosaccharides, disaccharides, monosaccharides, etc. with different molecular weights. Development and industrial production have decisive significance. At present, some people have used Vibrio sp, N-5 to obtain exo-fucosidase. Others use Bacillus and other microorganisms to produce fucosidase. However, it takes a long time to produce enzymes with these strains, the enzyme activity of the output is low, and the culture components are complicated, so it is difficult to be suitable for industrial production. Contents of the invention [0003] The object of the present invention is to provide a method for producing fucoidan sulfatase by Vibrio riparianus, which can overcome the above-mentioned shortcomings of the prior...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/00
Inventor 江晓路胡晓珂
Owner OCEAN UNIV OF CHINA
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