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Producing basic proteinase by vibrio metschnikovii DL 33-51 strain

A protease, alkaline technology, applied in bacteria, hydrolase, fermentation and other directions, can solve problems such as the decline of enzyme activity, and achieve the effect of short production cycle, good stability and wide range of nutritional requirements

Inactive Publication Date: 2005-01-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the optimum pH for the reaction of alkaline protease derived from microorganisms is generally 9.0-10.0, and various oxidants and surfactants can cause different degrees of enzyme activity decline

Method used

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  • Producing basic proteinase by vibrio metschnikovii DL 33-51 strain
  • Producing basic proteinase by vibrio metschnikovii DL 33-51 strain
  • Producing basic proteinase by vibrio metschnikovii DL 33-51 strain

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Embodiment Construction

[0014] Water samples were collected from the alkali lake, and the colony with a large transparent circle on the casein plate was selected, and a bacterial strain with high alkaline protease activity was obtained by measuring the enzyme activity of its fermentation broth for re-screening. The strain was identified as Vibrio mesoni in the genus Vibrio through morphological observation and physiological and biochemical identification. The composition of the casein medium is: 1.0g casein, 1.0g soybean flour, 14.0g NaCl, KH 2 PO 4 1.0g, CaCl 2 0.1g, agar 15.0g, H 2 O1000mL, pH10.0.

[0015] After bacterial classification was activated with casein medium, insert it into 100mL fermentation medium (casein 1.0g, soybean powder 1.0g, NaCl14.0g, KH 2 PO 4 1.0g, CaCl 2 0.1g, H 2 (21000mL, pH10.4) in a 250mL Erlenmeyer flask, cultured in a shaker flask at 26°C for 40h, and centrifuged at 3000rpm for 10 minutes at 4°C to remove the thallus to obtain a fermented enzyme liquid. Condit...

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Abstract

The invention relates to a microbe Vibrio metschnikovii Dl 33-51, able to generate basic proteinase with molecular weight of 29.5 kDa. It provides a strain Vibrio metschnikovii Dl 33-51 coming from the nature and a method of producing basic proteinase by this strain. It separates and purifies a strain Vibrio metschnikovii Dl 33-51 from soda lakes, which has a character of generating proteinase. By Bergey's Manual (the ninth edition), this strain has the shape and physiological and biochemical characteristics basically according with those of the Vibrio metschnikovi. This strain has the characters of wide nutrition demands, easily culturing and short culture time. And the generated basic proteinase has high activity. Use this strain as a strain for producing the basic proteinase has the characters of high activity, good pH value and thermal stability, ability to resist surfactant and oxidant. The basic proteinase can be developed into a new enzyme preparation applied in normal-temperature washing agent, implementing industrialized production.

Description

technical field [0001] The invention relates to a microorganism Vibrio metschnikovii DL 33-51 strain producing alkaline protease. Background technique [0002] According to the present inventor's knowledge through consulting data and literature search, the strains that produce alkaline protease are mainly derived from Bacillus and some fungi. Some strains of Bacillus, Streptomyces, Aspergillus, Pseudomonas can produce alkaline protease. According to the ninth edition of Bergey's Bacteria Identification Manual, most of the morphological and physiological and biochemical characteristics of the strain of the present invention are consistent with Vibrio metschnikovii of the genus Vibrio. [0003] Alkaline protease is mainly used as a detergent additive in industry, and it is required to maintain high enzyme activity and stability in a wide range of pH and temperature, and must also be fused with washing powder containing various oxidants and surfactants. At present, the optimu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/50C12P1/04C12P21/02
Inventor 江晓路梅承芳
Owner OCEAN UNIV OF CHINA
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