Anti-cancer active substance of cyathocline purpurea and separating and purifying method thereof

Abstract

The invention relates to anticancer active components of chrysanthemum chrysanthemum and a separation and purification method thereof. It is characterized in that the anti-cancer compound is 9β-acetyl-cerciolactone (CP-C), and the molecular formula is C 17 h 22 o 4 and 9β-acetyl-guayulelide (CP-D), the molecular formula is C 17 h 22 o 5 . The separation and purification method is as follows: dry and pulverize the whole plant of Chrysanthemum chrysanthemum, extract the extract, and obtain the extract CP 0 ; add diatomaceous earth and fry dry on a mixed water bath with ethanol, use petroleum ether, chloroform: ethyl acetate=1: 0~0: 1, ethyl acetate, acetone, methanol to extract 5 components successively; use L 1210 The cells were screened for cytotoxic activity of the 5 fractions, and the 3 fractions with anticancer activity were subjected to column chromatography to obtain 3 anticancer active ingredients. The separation and purification method adopted in the present invention is simple and feasible, has high yield and is unique. The obtained three anti-cancer active components (CP-B, CP-C, CP-D) have strong activity, which exceeds the international standard, and the raw materials of the cup chrysanthemum are easy to get (can be artificially cultivated), and have the prospect of being developed as a new anti-cancer drug .

Description

technical field The invention relates to the technical field of medical drugs, in particular to extracting anticancer active ingredients from Chrysanthemum chinensis and a separation and purification method thereof. Background technique Cup chrysanthemum is a plant of the genus Cyathocline Purpurea Buch.-Ham.ex De Don in the family Asteraceae. There are about 3 species of plants of the genus Cyathocline Purpurea in the world, and 1 species in China [Lin Rong, Chen Yilin. Flora of China (Seventh Fourteen volumes). Beijing: Science Press, 81-82]. Cup chrysanthemum is mainly distributed in the subtropical regions of Southwest China and India. The whole plant of Chrysanthemum chrysanthemum is used as medicine, and it is used by folks to reduce inflammation and stop bleeding, prevent and treat various inflammations, colds, and high fever. The domestic and foreign research overview of the chemical constituents of the genus Chrysanthemum: In 1981, Indian scholar Bhimsen A. N...

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Problems solved by technology

At present, there is no other report on the chemical constituents of the genus Chrysanthemum and its application

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