Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Coupled enzyme for skin care

A technology for skin care and enzyme modification, which is applied in the field of modified enzymes for skin care, and can solve problems such as interfering with polypeptide interactions and reducing polypeptide activity

Inactive Publication Date: 2000-05-17
NOVO NORDISK AS
View PDF26 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] It has been found that linking a polypeptide to a polymeric molecule often reduces the activity of the polypeptide or interferes with the interaction of the polypeptide with its substrate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Coupled enzyme for skin care
  • Coupled enzyme for skin care
  • Coupled enzyme for skin care

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0335] Activate mPEG with N-succinimide carbonate 15,000

[0336] mPEG 15,000 was suspended in toluene (4ml / g mPEG), and 20% was distilled off under normal pressure to azeotropically dry the reactant. When the solution was cooled to 30°C, dichloromethane (1 ml / g dry mPEG) was added, and phosgene (1.93M 5 mol / mol mPEG) in toluene was added and the mixture was stirred overnight at room temperature. The mixture was evaporated to dryness and the desired product was obtained as a waxy mass.

[0337] After evaporation, dichloromethane and toluene (1:2, 3ml / g dry mPEG) were added to redissolve the white solid. N-hydroxysuccinimide (2 mol / mol mPEG) was added as a solid, followed by triethylamine (1.1 mol / mol mPEG). The mixture was stirred for 3 hours. The mixture was not clear at first, then became clear and eventually a small precipitate formed. The mixture was evaporated to dryness, recrystallized from ethyl acetate (10ml) and filtered hot to remove salts and insoluble traces. ...

Embodiment 2

[0339] Activate mPEG with N-succinimide carbonate 5,000

[0340] Activation of mPEG 5,000 with N-succinimide carbonate was performed as described in Example 1.

Embodiment 3

[0342] Binding of PD498 protease to activated mPEG 5,000

[0343] In a final volume of 20 ml, 200 mg of PD498 was incubated with 1.8 g of mPEG 5,000 activated with N-succinimide carbonate (prepared according to Example 2) in 50 mM sodium borate, pH 10. The reaction was carried out at room temperature with magnetic stirring. The reaction time is 1 hour. By adding DMG buffer to a final concentration of 5 mM dimethyl glutarate, 1 mM CaCl 2 And 50mM borate (pH5.0) to stop the reaction.

[0344] The molecular weight of the resulting derivative was about 100 kDa, corresponding to about 13 moles of mPEG attached per mole of PD498.

[0345] Compared with the parent enzyme, the remaining activity of the modified enzyme on the peptide substrate (succinyl-Ala-Ala-Pro-Phe-acyl-p-nitroanilide) was close to 100%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to modified enzymes suitable for skin care having from 4 to 70 polymeric molecules, with a molecule weight from 1 to 35 kDa, coupled covalently to the surface of parent enzymes having a molecule weight from 15 to 100 kDa. Further the invention is directed towards skin care compositions and products comprising such modified enzymes and finally the use of said modified enzyme for reducing the sensitisation potential of skin care products.

Description

field of invention [0001] The present invention relates to modified enzymes, skin care compositions comprising said modified enzymes and ingredients known to be used in skin care compositions, skin care products comprising the skin care compositions of the present invention and said modified enzymes The use of modifying enzymes for improving the stability of enzymes and / or reducing the allergenic ability of enzymes. Background of the invention [0002] People have loved bathing and showering since ancient times. This has not changed so far. For most people today, bathing and showering are part of everyday life used to maintain good body hygiene and achieve a pleasant smell. Certain people also regard a refreshing shower or bath in the morning as an important and necessary spiritual experience without which they cannot wake up. [0003] Many products for body care and good body hygiene can be found on the consumer market, such as products for cleansing and moisturizing all...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/00A61K8/00A61K8/02A61K8/06A61K8/34A61K8/36A61K8/362A61K8/64A61K8/66A61K8/73A61K8/86A61K8/96A61Q1/00A61Q1/02A61Q1/04A61Q1/06A61Q1/10A61Q1/12A61Q1/14A61Q3/00A61Q3/04A61Q5/00A61Q5/02A61Q5/06A61Q5/10A61Q5/12A61Q7/00A61Q9/02A61Q15/00A61Q17/02A61Q17/04A61Q19/00A61Q19/02A61Q19/10C12N9/96
CPCA61K2800/57A61Q19/00A61K8/66A61Q5/06A61Q5/02A61Q5/10A61Q19/10C12N9/96A61Q9/02A61Q5/12A61K2800/70A61K8/86
Inventor A·A·奥尔森A·普伦特
Owner NOVO NORDISK AS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com