Air lift plant cell culture biological reactor with screen mesh guiding drum

A bioreactor and plant cell technology, which is applied in the field of airlift plant cell culture bioreactors, can solve the problems of metabolite generation affecting cell growth, the inoculation amount cannot be too large, and the plant cell damage is large, etc. Soluble, easy to operate, good for flow effect

Inactive Publication Date: 2006-05-03
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the shear force in the existing draft tube airlift reactor still causes great damage to plant cells, and there is no solid surface on which cells can attach, which affects the growth of cells and the generation of metabolites
In addition, when using the existing airlift bioreactor to cultivate plant cells, the inoculation amount should not be too large, otherwise the cells will easily brown and even die due to insufficient oxygen supply

Method used

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  • Air lift plant cell culture biological reactor with screen mesh guiding drum
  • Air lift plant cell culture biological reactor with screen mesh guiding drum
  • Air lift plant cell culture biological reactor with screen mesh guiding drum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Prepare 1 set of 2L screen-type draft tube air-lift plant cell culture bioreactor provided by the present invention, such as figure 1 As shown, a reactor body 1 made of φ120mm×300mm heat-resistant glass, the built-in light source 10 is fixed on the top cover of the reactor body along the axis of the reactor body 1, and a screen made of stainless steel mesh The type guide tube 2 is axially screwed and fixed on the bracket at the bottom of the reactor, and is located in the center of the reactor main body 1, and the circular gas distributor 3 located directly below the screen type guide tube 2 is fixed on the reactor main body 1 On the base plate, the circular gas distributor 3 communicates with the air inlet 5 on the base plate, and the air inlet 5 links to each other with an external air compressor, and an air filter 11 is installed on the connecting pipe, and a gas flowmeter 6 (with to adjust the size of the air flow), the reactor main body 1 is evenly equipped with an...

Embodiment 2

[0026] Prepare 1 set of 2L screen-type draft tube air-lift plant cell culture bioreactor provided by the present invention, such as figure 2 As shown, a reactor body 1 made of φ120mm×300mm heat-resistant glass, the built-in light source 10 is fixed on the top cover of the reactor body along the axis of the reactor body 1, and two diameters made of stainless steel mesh are respectively The 40mm and 80mm screen-type draft tubes 2 are coaxially set and fixed in the reactor main body 1, and an annular gas distributor 3 is arranged directly below the annular area of ​​the screen-type draft tube 2, and the annular gas distributor 3 is installed on the bottom plate of the reactor main body 1, and communicates with the air inlet 5, which is connected with an external air compressor, and an air filter 11, a gas temperature control device 13 and a gas flow meter 6 are installed on the connecting pipe, An external fluorescent tube 9 is evenly arranged around the reactor main body 1, and...

Embodiment 3

[0029] Prepare a 40L screen-type draft tube air-lift plant cell culture bioreactor, such as image 3 As shown, a reactor main body 1 made of heat-resistant glass of φ230mm×1400mm, three screen-type guide tubes 2 with diameters of 60mm, 120mm and 180mm made of stainless steel mesh are fixed on the reactor. In the reactor main body 1, the built-in light source 10 is fixed on the top cover of the reactor main body along the axis of the reactor main body 1. The built-in light source 10 is made by inserting a fluorescent tube into a φ40mm heat-resistant glass built-in light tube.

[0030] There is a circular gas distributor in the circular area directly below the screen-type draft tube located in the center, and there is an annular gas distributor 3 directly below the annular area between the two screen-type draft tubes on the periphery. The two gas distributors 3 are all installed on the bottom plate of the reactor main body 1, and communicate with the air inlet 5, which is connec...

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PUM

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Abstract

The invention relates to a screen-type draft tube airlift plant cell culture bioreactor, comprising a reactor main body, a draft tube, an external light source and a gas distributor, wherein the reactor main body is round or square and transparent The cylinder body and the guide tube are fixed in the main body of the reactor, and there is a distance between the upper and lower walls of the main body of the reactor. The upper wall of the main body of the reactor is provided with an inoculation port, an air outlet and a liquid replenishment port, and the lower wall is provided with There is an air inlet, which communicates with the gas distributor installed at the bottom of the reactor main body, and the external light source is evenly distributed around the reactor main body. It is characterized in that: the guide tube is made of metal mesh The screen-type draft tube is located at the center of the reactor body, and the gas distributor is located directly below the screen-type draft tube. The reactor allows plant cells to attach to the screen to grow, reduces the shearing force of the airflow on the plant cells, is suitable for large-scale cultivation of plant cells, and has the advantages of simple structure and convenient operation.

Description

technical field [0001] The invention belongs to a bioreactor used in the field of biotechnology, in particular to an air-lift plant cell culture bioreactor with a screen-type draft tube. Background technique [0002] A bioreactor is a reactor for cultivating microorganisms, plants and animal cells on a large scale, such as an external circulation bioreactor (W.W.Su, US5342781) suitable for culturing microorganisms, plants and animal cells; A bioreactor (K.Hermann, EP0155237) composed of reaction chambers (comprising cell culture chambers, culture fluid chambers and product chambers); a membrane bioreactor (Xu Youyi et al., CN1239709A) for the cultivation of nitrifying bacteria; A gas-liquid circulation bioreactor for single-cell protein production (Wang Hongqi et al., CN85109440A); a bioreactor for microbial cell culture (Sen Jing, CN87104667; Xu Xiaozeng, etc., CN1124292A). People have utilized bioreactors to cultivate plant cell tissues and organs to carry out industrial ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
Inventor 王玉春欧阳杰王晓东
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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